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4/20/2016 1:41:00 PM | Browse: 1307 | Download: 2338
Publication Name World Journal of Translational Medicine
Manuscript ID 22939
Country/Territory Italy
Received
2015-10-05 14:05
Peer-Review Started
2015-10-05 16:25
To Make the First Decision
2015-11-24 14:41
Return for Revision
2015-11-27 08:29
Revised
2015-12-14 23:39
Second Decision
2016-01-22 15:10
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2016-01-31 14:22
Articles in Press
2016-01-31 14:22
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2016-04-06 11:42
Publish the Manuscript Online
2016-04-11 10:17
ISSN 2220-6132 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Medicine, General & Internal
Manuscript Type Basic Study
Article Title Prednisolone inhibits SaOS2 osteosarcoma cell proliferation by activating inducible nitric oxide synthase
Manuscript Source Invited Manuscript
All Author List Alessandra Cazzaniga, Jeanette AM Maier and Sara Castiglioni
Funding Agency and Grant Number
Corresponding Author Dr. Jeanette AM Maier, Dipartimento di Scienze Biomediche e Cliniche Luigi Sacco, Università di Milano, via GB Grassi 74, 20157 Milano, Italy. jeanette.maier@unimi.it
Key Words Osteosarcoma cells; Prednisolone; Nitric oxide; Inducible nitric oxide synthase; Endothelial nitric oxide synthase; Reactive oxygen species
Core Tip Since prednisolone, a widely used synthetic glucocorticoid, inhibits osteoblast proliferation, we evaluated its effects on osteosarcoma cells. In particular, we used two osteoblastic osteosarcoma cell lines with different degree of differentiation, i.e., SaOS2, which have a rather mature phenotype, and U2OS, which are less differentiated. We found that prednisolone inhibited SaOS2 proliferation by increasing the release of nitric oxide (NO) through the upregulation of inducible NO synthase (iNOS). Indeed, pharmacological inhibition with the iNOS inhibitor l-N6-(iminoethyl)-lysine-HCl restored the normal pro-liferation rate of the SaOS2. On the contrary, predni-solone did not modulate NO production nor cell growth in U2OS.
Publish Date 2016-04-11 10:17
Citation Cazzaniga A, Maier JAM, Castiglioni S. Prednisolone inhibits SaOS2 osteosarcoma cell proliferation by activating inducible nitric oxide synthase. World J Transl Med 2016; 5(1): 53-58
URL http://www.wjgnet.com/2220-6132/full/v5/i1/53.htm
DOI http://dx.doi.org/10.5528/wjtm.v5.i1.53
Full Article (PDF) WJTM-5-53.pdf
Full Article (Word) WJTM-5-53.doc
Manuscript File 22939-Review.docx
Answering Reviewers 22939-Answering reviewers.pdf
Audio Core Tip 22939-Audio core tip.mp3
Biostatistics Review Certificate 22939-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 22939-Conflict-of-interest statement.pdf
Copyright License Agreement 22939-Copyright assignment.pdf
Institutional Animal Care and Use Committee Approval Form or Document Institutional_Animal_Care_and_Use_Committee_Statement_20151005140401.pdf
Institutional Review Board Approval Form or Document 22939-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 22939-Language certificate.pdf
Supplementary Material Supplementary_Material_20151005140415.pdf
Peer-review Report 22939-Peer-review(s).pdf
Scientific Misconduct Check 22939-Scientific misconduct check.pdf
Scientific Editor Work List 22939-Scientific editor work list.pdf