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Articles Published Processes
2/9/2015 5:58:00 PM | Browse: 893 | Download: 1047
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Received |
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2014-07-13 12:00 |
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Peer-Review Started |
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2014-07-13 12:04 |
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To Make the First Decision |
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2014-08-15 11:56 |
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Return for Revision |
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2014-09-17 10:04 |
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Revised |
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Second Decision |
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2014-10-21 17:39 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Company Editor-in-Chief |
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2014-10-21 17:55 |
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Articles in Press |
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2014-10-21 18:10 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2014-12-07 21:05 |
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Typeset the Manuscript |
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2015-01-21 08:56 |
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Publish the Manuscript Online |
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2015-02-09 17:58 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
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| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Autobiography |
| Article Title |
Crude extract of maggots: Antibacterial effects against Escherichia coli, underlying mechanisms, separation and purification
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Quan-Sheng Ge, Hui-Min Zhang, Xia Liu, Shou-Yu Wang, De-Cheng Lv and Xu-Dong Li |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation of China |
81270052 |
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| Corresponding Author |
Dr. Hui-Min Zhang, the First Affiliated Hospital of Dalian Medical University, No. 222 Zhongshan Road, Xiagang District, Dalian 116011, Liaoning Province, China. quansheng0618@163.com |
| Key Words |
Maggots; Antibacterial peptide; Antibacterial mechanism; Escherichia coli; Colorectal |
| Core Tip |
Previous studies have found the antibacterial activity of the maggots, but studies on the underlying mechanisms are still lacking. In this study, we separated and purified the crude extract of maggots using DEAE-Sepharose ion exchange chromatography and Sephacryls-200HR gel filtration chromatography. Agarose gel electrophoresis and fluorescence probe technique were used to investigate the antibacterial mechanism of the crude extract of maggots. We found that the protein of maggots weighing around 15 KD had the antibacterial effect and the crude extracts played the antibacterial role by destroying the cell membrane of Escherichia coli and disturbing the DNA replication of plasmids. |
| Publish Date |
2015-02-09 17:58 |
| Citation |
Ge QS, Zhang HM, Liu X, Wang SY, Lv DC, Li XD. Crude extract of maggots: Antibacterial effects against Escherichia coli, underlying mechanisms, separation and purification. World J Gastroenterol 2015; 21(5): 1510-1517 |
| URL |
http://www.wjgnet.com/1007-9327/full/v21/i5/1510.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v21.i5.1510 |
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