Publication Name	World Journal of Gastroenterology
Manuscript ID	1497
Country	China

Received	2012-12-16 09:52
Peer-Review Started	2012-12-17 17:31
To Make the First Decision	2013-01-17 11:04
Return for Revision	2013-01-23 20:30
Revised	2013-02-01 23:53
Second Decision	2013-03-22 11:59
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2013-03-23 09:19
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2013-06-06 07:56
Publish the Manuscript Online	2013-06-28 23:55

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Gastroenterology & Hepatology
Manuscript Type	Autobiography
Article Title	Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells
Manuscript Source	Invited Manuscript
All Author List	Jian Zou, Lin Mi, Xiao-Feng Yu and Jie Dong
Funding Agency and Grant Number	Funding Agency Grant Number Medical Guidance Projects of Shanghai Science Committee 10411961800 National Natural Science Foundation of China 81101617
Corresponding Author	Xiao-Feng Yu, Professor, Department of Gastroenterology, Huadong Hospital, Fudan University, 221 Yan an Xi Road, Shanghai 200040, China. zoujian-dj@163.com
Key Words	14-3-3σ protein; Interacting proteins; Yeast two-hybrid system; Colon cancer stem cells
Core Tip	Of the 14-3-3 proteins, tumor-suppressor activity has most clearly been defined for 14-3-3σ. In the study, we constructed 14-3-3σ bait gene and expressed as a fusion to the GAL4 DNA-binding domain successfully. Using the yeast two-hybrid system, we found novel binding proteins from the HeLa cDNA library which closely interact with 14-3-3σ. Our results also suggest that 14-3-3σ may interact with potassium channel modulatory factor 1 (KCMF1) protein. The knock-down expression of 14-3-3σ and KCMF1 proteins significantly inhibited proliferation and colony formation of SW1116csc cells. So, 14-3-3σ and other proteins may be involved in proliferation and colony formation of human colon cancer stem cells.
Publish Date	2013-06-28 23:55
Citation	Zou J, Mi L, Yu XF, Dong J. Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells. World J Gastroenterol 2013; 19(24): 3770-3780
URL	http://www.wjgnet.com/1007-9327/full/v19/i24/3770.htm
DOI	http://dx.doi.org/10.3748/wjg.v19.i24.3770

Full Article (PDF)	WJG-19-3770.pdf
Manuscript File	1497-Review.doc
Answering Reviewers	1497-Answering reviewers.doc
Copyright License Agreement	1497-Copyright assignment.doc
Non-Native Speakers of English Editing Certificate	1497-Language certificate.pdf
Peer-review Report	1497-Peer review(s).pdf
Scientific Editor Work List	1497-Scientific editor work list.doc