Publication Name	World Journal of Stem Cells
Manuscript ID	3264
Country	Spain

Received	2013-04-18 08:18
Peer-Review Started	2013-04-18 16:22
To Make the First Decision	2013-05-14 18:44
Return for Revision	2013-05-17 11:35
Revised	2013-06-11 18:27
Second Decision	2013-08-13 11:46
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2013-08-13 13:31
Articles in Press
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2013-09-11 13:43
Publish the Manuscript Online	2013-10-31 14:50

ISSN	1948-0210 (online)
Open Access
Copyright
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Website	http://www.wjgnet.com

Category	Hematology
Manuscript Type	Autobiography
Article Title	Human adipose tissue contains erythroid progenitors expressing fetal hemoglobin
Manuscript Source	Invited Manuscript
All Author List	Amparo Navarro, Francisco Carbonell-Uberos, Severiano Marín and María Dolores Miñana
Funding Agency and Grant Number	Funding Agency Grant Number Ministerio de Ciencia e Innovación PI08/1716 Ministerio de Sanidad y Consumo EMER07/005 Consellería de Sanidad AP061/09 Generalitat Valenciana AP069/10
Corresponding Author	Dr. María Dolores Miñana, PhD, Regenerative Medicine Laboratory, Fundación Hospital General Universitario, Ave. Tres Cruces s/n, 46014 Valencia, Spain. minyana_mdo@gva.es
Key Words	Hemoglobin; Adipose tissue; Stromal vascular fraction; Erythroid cells; Hematopoietic progenitors
Core Tip	Stromal vascular fraction (SVF) from human adipose tissue contains mesodermal precursors with the ability to form mixed hematoendothelial colonies and hematopoietic colony-forming units, though this occurs at an extremely low frequency. It is well known that hematopoietic progenitors residing in the bone marrow are released into the circulation and enter peripheral tissues; therefore, the most plausible explanation for this hematopoietic activity is that these cells are actually circulating hematopoietic progenitors. However, it is also possible that they may originate from the adipose tissue itself. To address this hypothesis, we compared the expression levels of the most relevant hematopoietic transcription factors in cells isolated from SVF with their expression levels in CD34+ cells isolated from adult peripheral blood and cord blood. Moreover, because the composition of hemoglobin in erythroid cells varies depending on the origin of the hematopoietic progenitors and their ontogenic stage, burst-forming units-erythroid were developed in culture, and the hemoglobin composition and globin gene expression in erythroid colonies were determined. Our results provide evidence that erythroid progenitors contained in SVF exhibit features that differ from those of circulating progenitors. These findings should encourage further research on stem cells and the microenvironment of human adipose tissue.
Publish Date	2013-10-31 14:50
Citation	Navarro A, Carbonell-Uberos F, Marín S, Mi?ana MD. Human adipose tissue contains erythroid progenitors expressing fetal hemoglobin. World J Stem Cells 2013; 5(4): 205-216
URL	http://www.wjgnet.com/1948-0210/full/v5/i4/205.htm
DOI	http://dx.doi.org/10.4252/wjsc.v5.i4.205

Full Article (PDF)	WJSC-5-205.pdf
Manuscript File	3264-Review.doc
Answering Reviewers	3264-Answering reviewers.pdf
Copyright License Agreement	3264-Copyright assignment.pdf
Institutional Review Board Approval Form or Document	Ethics comittee approval document.doc
Non-Native Speakers of English Editing Certificate	3264-Language certificate.pdf
Peer-review Report	3264-Peer reviews.pdf
Scientific Editor Work List	3264-Scientific editor work list.doc