ISSN |
1948-0210 (online) |
Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
Copyright |
© The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. |
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Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
Website |
http://www.wjgnet.com |
Category |
Cell Biology |
Manuscript Type |
Basic Study |
Article Title |
Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential
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Manuscript Source |
Unsolicited Manuscript |
All Author List |
Harumichi Itoh, Shimpei Nishikawa, Tomoya Haraguchi, Yu Arikawa, Shotaro Eto, Masato Hiyama, Toshie Iseri, Yoshiki Itoh, Munekazu Nakaichi, Yusuke Sakai, Kenji Tani, Yasuho Taura and Kazuhito Itamoto |
ORCID |
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Funding Agency and Grant Number |
Funding Agency |
Grant Number |
JSPS KAKENHI (Grants-in-Aid for Scientific Research; grant No. 26893172) |
to Nishikawa S |
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Corresponding Author |
Shimpei Nishikawa, PhD, Department of Small Animal Clinical Science, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8511, Japan. sn2007@yamaguchi-u.ac.jp |
Key Words |
Adipose-derived stem/stromal cell; Aldehyde dehydrogenase activity; Flow cytometry; Subpopulation; Ribosome |
Core Tip |
Aldehyde dehydrogenase (ALDH) activity is widely used as a stem cell marker in several types of normal or malignant tissues. However, there was no report of ALDH activity in murine adipose-derived stem cells (ADSCs). Here, our study demonstrated a subpopulation defined by high ALDH activity within murine ADSCs. The subpopulation with high ALDH activity (ALDHHi) showed enhanced differentiation potentials into adipocyte and osteocyte. Furthermore, gene set enrichment analysis revealed that ribosome-related gene sets were enriched in ALDHHi of murine ADSCs. We showed relationship between ALDHHi and ribosome biosynthesis, providing a novel insight of mesenchymal stem cell biology. |
Publish Date |
2017-10-24 02:59 |
Citation |
Itoh H, Nishikawa S, Haraguchi T, Arikawa Y, Eto S, Hiyama M, Iseri T, Itoh Y, Nakaichi M, Sakai Y, Tani K, Taura Y, Itamoto K. Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential. World J Stem Cells 2017; 9(10): 179-186 |
URL |
http://www.wjgnet.com/1948-0210/full/v9/i10/179.htm |
DOI |
http://dx.doi.org/10.4252/wjsc.v9.i10.179 |