Publication Name	World Journal of Gastrointestinal Oncology
Manuscript ID	109378
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Rapamycin suppresses small bowel adenocarcinoma HUTU 80 cells proliferation by inhibiting hypoxia-inducible factor-1α mediated metabolic reprogramming
Manuscript Source	Unsolicited Manuscript
All Author List	Bao-Peng Pu, Peng-Hui Wang, Kai-Kai Guo, Chun Liu, Si-Run Chen, Xiao-Meng Li, Shi-Min Chen, Xiang-Zhou Zeng and Chang Gao
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China No. 81660270 Hainan Provincial Natural Science Foundation of China No. 823RC497 Project of Nanhai Series of Talent Cultivation Program No. 20192031 Key Discipline Project of Pathophysiology at Hainan Medical University No. 05 The Open Project Fund for Provincial Key Disciplines of Basic Medicine at Hainan Medical University, Hainan Medical College Talent Research Start up Fund No. RZ300006194
Corresponding Author	Chang Gao, Assistant Professor, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory of Carcinogenesis and Intervention, School of Basic Medicine and Life Sciences, Hainan Medical University, No. 3 Academy Road, Haikou 571199, Hainan Province, China. gaochang@muhn.edu.cn
Key Words	Rapamycin; Small bowel adenocarcinoma; Mammalian target of rapamycin/hypoxia-inducible facto-1α; Metabolic reprogramming; Warburg effect; Cell cycle arrest; Xenograft
Core Tip	Rapamycin is an immunosuppressive agent that inhibits the proliferation of tumor cells. HUTU 80 cells were used as a representative model for small bowel adenocarcinoma (SBA). Rapamycin inhibited the proliferation, migration, and invasion of HUTU 80 cells and downregulated glycolysis levels and cell cycle arrest. Transcriptomic and metabolomic analyses were used to find the potential targets of action of rapamycin in inhibiting HUTU 80 cell proliferation. The key mechanism of rapamycin against SBA is related to the mammalian target of rapamycin/70 kDa ribosomal protein S6 kinase/4E-binding protein 1/hypoxia-inducible factor-1α signaling pathway.
Citation	Pu BP, Wang PH, Guo KK, Liu C, Chen SR, Li XM, Chen SM, Zeng XZ, Gao C. Rapamycin suppresses small bowel adenocarcinoma HUTU 80 cells proliferation by inhibiting hypoxia-inducible factor-1α mediated metabolic reprogramming. World J Gastrointest Oncol 2025; In press

Received	2025-05-09 10:39
Peer-Review Started	2025-05-09 10:40
To Make the First Decision
Return for Revision	2025-05-27 04:54
Revised	2025-06-20 06:44
Second Decision	2025-08-18 02:35
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-08-18 07:19
Articles in Press	2025-08-18 07:19
Publication Fee Transferred	2025-06-22 04:02
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-5204 (online)
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