Publication Name	World Journal of Gastroenterology
Manuscript ID	111706
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Thymosin β4 released by mast cells under stress conditions impairs intestinal epithelial barrier via IL22RA1/JAK1/STAT3 signaling in irritable bowel syndrome
Manuscript Source	Unsolicited Manuscript
All Author List	Yue-Shan Sun, Xiao-Qin Bai, Kai-Di Sun, Jiao Li, Lei Liu, Yuan-Yuan Chen, Zhao-Yu Zeng, Qiong Wang and Yuan-Biao Guo
Funding Agency and Grant Number	Funding Agency Grant Number the National Natural Science Foundation of China 81270465 the Sichuan Science and Technology Program 2024NSFSC0631, 2023JDRC0088, MZGC20240097 the Basic Research Cultivation Support Program of Fundamental Research Funds for the Central Universities 2682023ZTPY071 the Baiqiuen Public Welfare Foundation Program BCF-LX-XH-20221014-12 the Third People’s Hospital of Chengdu Clinical Research Program CSY-YN-01-2023-012 and CSY-YN-03-2024-013 the Yibin Science and Technology Program 2021ZYY009 the Chengdu Medical Research Project Foundation 2022284
Corresponding Author	Yuan-Biao Guo, PhD, Medical Research Center, The Third People's Hospital of Chengdu, No. 82 Qinglong Street, Qingyang District, Chengdu 610031, Sichuan Province, China. guoyuanbiao@swjtu.edu.cn
Key Words	Thymosin β4; Intestinal barrier; Mast cell; Janus kinase 1/signal transducer and activation of transcription 3; Irritable bowel syndrome
Core Tip	Thymosin β4 (Tβ4) is identified as a prevalent component in the intestinal mucus of irritable bowel syndrome patients that is capable of independently causing intestinal barrier disruption, even without the involvement of mast cells, when triggered by either corticotropin-releasing hormone (CRH) or stress. In addition, mast cells are the source of the Tβ4 required for intestinal barrier damage, and its release is contingent on CRH/CRHR1 but not on degranulation. Mechanistically, the impact of Tβ4 on both the physical and immune barriers is mediated through the inhibition of Janus kinase 1/signal transducer and activation of transcription 3 signaling. These findings position Tβ4 as a promising diagnostic biomarker and therapeutic target for irritable bowel syndrome.
Citation	Sun YS, Bai XQ, Sun KD, Li J, Liu L, Chen YY, Zeng ZY, Wang Q, Guo YB. Thymosin β4 released by mast cells under stress conditions impairs intestinal epithelial barrier via IL22RA1/JAK1/STAT3 signaling in irritable bowel syndrome. World J Gastroenterol 2025; In press

Received	2025-07-08 01:46
Peer-Review Started	2025-07-08 01:46
To Make the First Decision
Return for Revision	2025-08-27 08:39
Revised	2025-09-04 14:16
Second Decision	2025-10-13 02:50
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-10-13 07:37
Articles in Press	2025-10-13 07:37
Publication Fee Transferred	2025-09-05 13:32
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Typeset the Manuscript

ISSN	1007-9327 (print) and 2219-2840 (online)
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