Publication Name	World Journal of Stem Cells
Manuscript ID	113614
Country	Taiwan

Category	Obstetrics & Gynecology
Manuscript Type	Basic Study
Article Title	Extracellular vesicles derived from human amniotic fluid stem cells improve bladder dysfunction in rat model of diabetic atherosclerosis
Manuscript Source	Unsolicited Manuscript
All Author List	Ching-Chung Liang, Yi-Hao Lin, Chu-Ya Liang, Aileen Ro, Yung-Hsin Huang and Steven W Shaw
Funding Agency and Grant Number	Funding Agency Grant Number Ministry of Science and Technology Taiwan MOST 109-2314-B-182A-091 Ministry of Science and Technology Taiwan NSTC 112-2314-B-182A-062 Ministry of Science and Technology Taiwan NSTC 113-2314-B-182A-125
Corresponding Author	Steven W Shaw, Professor, Division of Obstetrics, Department of Obstetrics and Gynecology, Taipei Chang Gung Memorial Hospital, No. 199 Dunhua North Road, Taipei 105, Taiwan. doctor.obsgyn@gmail.com
Key Words	Atherosclerosis; Bladder; Diabetes; Exosome; Extracellular vesicles; Stem cell
Core Tip	In addition to cardiovascular complications, bladder dysfunction is one of the common comorbidities associated with diabetes, but is often refractory to current treatments. Human amniotic fluid stem cells (hAFSCs) demonstrated the efficacy in preclinical studies of diabetic bladder dysfunction and arterial atherosclerosis-induced bladder dysfunction. Here, we demonstrate that extracellular vesicles derived from hAFSCs could help restore diabetic atherosclerosis-induced bladder dysfunction, which is associated with lowered blood glucose levels, reduced arterial wall thickness, and decreased tumor necrosis factor-α, interleukin-6, transforming growth factor-β1, Smad3, and connective tissue growth factor expression. Our study highlights the potential of extracellular vesicles derived from hAFSCs in cell-free regenerative therapy of diabetic atherosclerosis.
Citation	Liang CC, Lin YH, Liang CY, Ro A, Huang YH, Shaw SW. Extracellular vesicles derived from human amniotic fluid stem cells improve bladder dysfunction in rat model of diabetic atherosclerosis. World J Stem Cells 2025; In press

Received	2025-09-01 09:54
Peer-Review Started	2025-09-01 09:56
To Make the First Decision
Return for Revision	2025-09-22 09:14
Revised	2025-10-06 04:05
Second Decision	2025-11-20 02:28
Accepted by Journal Editor-in-Chief	2025-11-25 23:25
Accepted by Executive Editor-in-Chief	2025-11-26 07:14
Articles in Press	2025-11-26 07:14
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ISSN	1948-0210 (online)
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