Publication Name	World Journal of Gastroenterology
Manuscript ID	114420
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Synergistic anti-hepatoma effect of triptolide and quercetin via co-inhibition and interaction with Janus kinase and mammalian target of rapamycin signal pathway
Manuscript Source	Unsolicited Manuscript
All Author List	Hong-Xuan Tong, Jia-Le Zhang, Wen-Yi Nie, Li-Jie Jiang, Jing-Qing Hu and Tao Lu
Funding Agency and Grant Number	Funding Agency Grant Number Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences CI2023C060YLL Fundamental Research Funds for the Central Public Welfare Research Institutes, China Academy of Chinese Medical Sciences YZX-202240, CYZ-202504
Corresponding Author	Tao Lu, PhD, Professor, School of Life Sciences, Beijing University of Chinese Medicine, No. 11 North 3rd Ring East Road, Chaoyang District, Beijing 100029, China. taolu@bucm.edu.cn
Key Words	Triptolide; Quercetin; Synergistic effect; Janus kinase signal pathway; Mammalian target of rapamycin signal pathway
Core Tip	This study demonstrates that a specific combination of triptolide (25 nmol/L) and quercetin (100 μmol/L) exerts a synergistic antitumor effect against hepatocellular carcinoma both in vitro and in vivo. The combination significantly inhibits tumor cell proliferation, induces apoptosis, and suppresses migration and invasion more effectively than either drug alone. Mechanistically, this synergy is achieved through the simultaneous inhibition of the Janus kinase-signal transducer and activator of transcription and mammalian target of rapamycin signaling pathways, as confirmed by transcriptomic analysis, molecular docking, and western blot validation. Importantly, the combination reduces the required dose of triptolide, thereby mitigating its toxic side effects while maintaining potent anticancer efficacy. This research highlights a promising therapeutic strategy for hepatocellular carcinoma that leverages the complementary actions of natural compounds to enhance treatment outcomes.
Citation	Tong HX, Zhang JL, Nie WY, Jiang LJ, Hu JQ, Lu T. Synergistic anti-hepatoma effect of triptolide and quercetin via co-inhibition and interaction with Janus kinase and mammalian target of rapamycin signal pathway. World J Gastroenterol 2025; In press

Received	2025-09-19 09:25
Peer-Review Started	2025-09-19 09:28
First Decision by Editorial Office Director	2025-10-13 09:32
Return for Revision	2025-10-13 09:32
Revised	2025-10-28 12:03
Publication Fee Transferred	2025-11-06 05:39
Second Decision by Editor	2025-12-08 02:34
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2025-12-08 07:15
Articles in Press	2025-12-08 07:15
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1007-9327 (print) and 2219-2840 (online)
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