Publication Name	World Journal of Gastrointestinal Oncology
Manuscript ID	114708
Country	United States

Category	Oncology
Manuscript Type	Editorial
Article Title	Revisiting multi-region 16S sequencing in gastric cancer
Manuscript Source	Invited Manuscript
All Author List	Liu Luo, Gang Huang, Hua Yang and Hao Chi
Funding Agency and Grant Number
Corresponding Author	Hao Chi, MD, Department of Quantitative Health Sciences, John A., Burns School of Medicine, University of Hawaii at Manoa,, 651 Ilalo Street, Honolulu, HI 96813, United States. chihao7511@gmail.com
Key Words	Gastric cancer; Microbiome; Multi-region 16S rRNA sequencing; Metagenomics; Biomarkers; Prognosis; Immune microenvironment; Precision oncology
Core Tip	Multi-region 16S rRNA sequencing represents a promising advance in gastric cancer microbiome research, improving microbial detection and taxonomic resolution compared to traditional methods. However, its clinical utility hinges on rigorous validation, integration with metagenomics and host factors, and the inclusion of health economic analyses. Future studies should aim to transform this technology from descriptive microbial profiling into a decision-support tool for prognosis, perioperative risk management, and personalized therapeutic strategies, overcoming existing methodological and translational challenges.
Citation	Luo L, Huang G, Yang H, Chi H. Revisiting multi-region 16S sequencing in gastric cancer. World J Gastrointest Oncol 2025; In press

Received	2025-09-26 07:09
Peer-Review Started	2025-09-26 07:09
To Make the First Decision
Return for Revision	2025-10-31 08:27
Revised	2025-11-08 11:16
Second Decision	2025-11-27 02:41
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-11-27 06:38
Articles in Press	2025-11-27 06:38
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-5204 (online)
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