| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Basic Study |
| Article Title |
Morphological and functional responses of three-dimensional-cultured colorectal cancer spheres to anticancer drugs
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Yusuke Yoshimura, Seiichi Shinji, Yutaro Ogawa, Takeshi Yamada, Akihisa Matsuda, Kay Uehara, Yasuyuki Yokoyama, Goro Takahashi, Takuma Iwai, Toshimitsu Miyasaka, Shintaro Kanaka, Takanori Matsui, Koki Hayashi, Masakazu Fujiwara, Yuuki Shichi, Tomio Arai, Toshiyuki Ishiwata and Hiroshi Yoshida |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| the JSPS KAKENHI (Grants-in-Aid for Scientific Research C) |
21K08744 |
| the JSPS KAKENHI (Grants-in-Aid for Scientific Research C) |
24K11881 |
| the JSPS KAKENHI (Grants-in-Aid for Scientific Research C) |
25K12018 |
| the JSPS KAKENHI (Grants-in-Aid for Scientific Research C) |
22K08882 |
| the JSPS KAKENHI (Grants-in-Aid for Scientific Research C) |
22K08835 |
|
| Corresponding Author |
Seiichi Shinji, Associate Professor, FACS, MD, PhD, Department of Gastroenterological Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku 113-8603, Tokyo, Japan. s-shinji@nms.ac.jp |
| Key Words |
Colorectal cancer; Sphere; Three-dimensional culture; 5-fluoride; Irinotecan; Oxaliplatin; Adenosine triphosphate/ATP assay; Water-soluble tetrazolium 8 assay; Quantitative reverse transcriptase PCR |
| Core Tip |
This study analyzed the effects of 5-fluorouracil, irinotecan, and oxaliplatin on three-dimensionally cultured colorectal adenocarcinoma (CRAC) spheres. Using cell viability assays, apoptosis detection, and quantitative reverse transcriptase PCR, we found that the residual spheres displayed increased expression of cancer stem cell and epithelial-mesenchymal transition markers, with distinct profiles between epithelial (HCT-15) and mesenchymal (SW480) types. These results indicate that CRAC spheres that survive anticancer drug treatment acquire hybrid epithelial-mesenchymal phenotypes and stem cell-like features, providing insights into the mechanisms of drug tolerance and cancer recurrence in colorectal cancer. |
| Citation |
Yoshimura Y, Shinji S, Ogawa Y, Yamada T, Matsuda A, Uehara K, Yokoyama Y, Takahashi G, Iwai T, Miyasaka T, Kanaka S, Matsui T, Hayashi K, Fujiwara M, Shichi Y, Arai T, Ishiwata T, Yoshida H. Morphological and functional responses of three-dimensional-cultured colorectal cancer spheres to anticancer drugs. World J Gastrointest Oncol 2026; In press |
| ISSN |
1948-5204 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2026. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
|
| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
