Publication Name	World Journal of Stem Cells
Manuscript ID	116388
Country	Pakistan

Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Maximizing pediatric stem cell harvest: A novel two-step digestion approach for adipose tissue
Manuscript Source	Invited Manuscript
All Author List	Asad Mushtaq, Mahmood S Choudhery, Taqdees Arif, Ahmad Niaz and Asim Pervaiz
Funding Agency and Grant Number
Corresponding Author	Mahmood S Choudhery, Department of Human Genetics and Molecular Biology, University of Health Sciences, Khayaban-e-Jamia Punjab, Lahore 54000, Punjab, Pakistan. ms20031@yahoo.com
Key Words	Stromal vascular fraction; Adipose tissue derived stem cells; Adipose tissue; Regenerative medicine; Two-step digestion approach
Core Tip	This study presents a novel dual-digestion method to isolate stem cells from small adipose tissue samples, optimizing cell yield for regenerative medicine applications. Traditionally, only the first stromal vascular fraction is retained for research or therapeutic purpose, while remaining undigested tissue is discarded. However, a second enzymatic digestion (yielding stromal vascular fraction 2) revealed a significant number of viable regenerative cells. They presence of viable regenerative cells was confirmed by plating efficiency, flow cytometry, gene expression analysis and histological studies. This approach is especially beneficial for pediatric patients or individuals with limited adipose reserves, enhancing the number of autologous stem cell by maximizing the utility of small-volume adipose tissue samples.
Citation	Mushtaq A, Choudhery MS, Arif T, Niaz A, Pervaiz A. Maximizing pediatric stem cell harvest: A novel two-step digestion approach for adipose tissue. World J Stem Cells 2026; In press

Received	2025-11-10 14:36
Peer-Review Started	2025-11-11 03:47
First Decision by Editorial Office Director	2025-12-04 08:52
Return for Revision	2025-12-04 08:52
Revised	2025-12-11 07:41
Publication Fee Transferred
Second Decision by Editor	2026-02-06 03:00
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2026-02-09 09:53
Articles in Press	2026-02-09 09:53
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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