Publication Name	World Journal of Stem Cells
Manuscript ID	120206
Country	China

Category	Cell Biology
Manuscript Type	Opinion Review
Article Title	Maximizing stem cell yield from limited adipose tissue: A novel two-step digestion approach and future artificial intelligence-integrated perspectives
Manuscript Source	Invited Manuscript
All Author List	Yi-Fan Wu, Xu Jiang, Jian-Guang Zhang and Wei Zhang
Funding Agency and Grant Number
Corresponding Author	Wei Zhang, PhD, Postdoc, Doctoral Workstation, Guangdong Eco-Engineering Polytechnic, No. 297 Guangshan First Road, Tianhe District, Guangzhou 510520, Guangdong Province, China. zw0915@163.com
Key Words	Stromal vascular fraction; Adipose-derived stem cells; Enzymatic digestion; Cell yield; Regenerative medicine; Pediatric applications; Artificial intelligence
Core Tip	The residual adipose tissue typically discarded after conventional enzymatic digestion contains viable and functionally-potent regenerative cells. The second digestion step yielded stromal vascular fraction 2, which exhibited high clonogenic potential. Although promising, the study’s generalizability is limited by its small sample size and lack of donor diversity. This review critically evaluates the methodology, discusses the impact of donor variability and enzyme lots on reproducibility, and proposes an artificial-intelligence-driven framework for personalizing digestion protocols. These insights aim to accelerate the clinical adoption and optimization of this valuable technique.
Citation	Wu YF, Jiang X, Zhang JG, Zhang W. Maximizing stem cell yield from limited adipose tissue: A novel two-step digestion approach and future artificial intelligence-integrated perspectives. World J Stem Cells 2026; In press

Received	2026-02-24 01:20
Peer-Review Started	2026-02-24 01:22
First Decision by Editorial Office Director	2026-03-13 09:10
Return for Revision	2026-03-13 09:10
Revised	2026-04-08 09:08
Publication Fee Transferred
Second Decision by Editor	2026-05-12 02:38
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2026-05-12 11:13
Articles in Press	2026-05-12 11:13
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-0210 (online)
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Copyright	©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc.
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