Publication Name	World Journal of Methodology
Manuscript ID	120686
DOI	10.5662/wjm.120686
Country	Japan

Category	Biochemical Research Methods
Manuscript Type	Minireviews
Article Title	Ultrasensitive enzyme-linked immunosorbent assay detection of proteins in membrane and lumenal fractions of small extracellular vesicles
Manuscript Source	Invited Manuscript
All Author List	Akane Sato and Etsuro Ito
Funding Agency and Grant Number	Funding Agency Grant Number Collaborative research grant from BioPhenoMA Inc
Corresponding Author	Etsuro Ito, Professor, Department of Biology, Waseda University, TWIns, 2-2 Wakamatsucho, Shinjuku 162-0056, Tōkyō, Japan. eito@waseda.jp
Key Words	78-kDa glucose-regulated protein; Biomarker; Membrane-lumen separation; Small extracellular vesicle; Ultrasensitive enzyme-linked immunosorbent assay
Core Tip	Analyzing the very low abundance of proteins contained in small extracellular vesicles is technically challenging, particularly when proteins are further separated into membrane and lumenal fractions. This review describes a practical method for fractionating small extracellular vesicles into membrane and lumenal components and introduces an ultrasensitive assay capable of quantifying trace amounts of proteins in each fraction through the combined use of commercially available kits.
Citation	Sato A, Ito E. Ultrasensitive enzyme-linked immunosorbent assay detection of proteins in membrane and lumenal fractions of small extracellular vesicles. World J Methodol 2026; In press DOI: https://doi.org/10.5662/wjm.120686
PDF	120686-in-press.pdf

Received	2026-03-06 02:23
Peer-Review Started	2026-03-06 02:23
First Decision by Editorial Office Director	2026-04-22 10:53
Return for Revision	2026-04-22 10:53
Revised	2026-05-11 07:25
Publication Fee Transferred
Second Decision by Editor	2026-06-23 02:18
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2026-06-23 06:49
Articles in Press	2026-06-23 06:49
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	2222-0682 (online)
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Copyright	©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc.
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