Publication Name	World Journal of Biological Chemistry
Manuscript ID	121018
DOI	10.4331/wjbc.121018
Country	United Kingdom

Category	Transplantation
Manuscript Type	Basic Study
Article Title	Improved stabilisation of human pancreas biopsies: Comparative evaluation of RNA preservation methods
Manuscript Source	Invited Manuscript
All Author List	Rhys R Pook, Abbie Hearne, Rachel A Botting, Samuel J Tingle, Minna Honkanen-Scott, James A M Shaw, Simi Ali and William E Scott III
Funding Agency and Grant Number	Funding Agency Grant Number National Institute for Health and Care Research Blood and Transplant Research Unit in Organ Donation and Transplantation No. NIHR203332
Corresponding Author	William E Scott III, Associate Professor, PhD, Department of Regenerative Medicine, Stem Cells, and Transplantation, Translational and Clinical Research Institute, Newcastle University, E1.08 Biomedicine West Wing, The International Centre for Life, Times Square, Scotswood Road, Newcastle upon Tyne NE2 4HH, England, United Kingdom. bill.scott3@newcastle.ac.uk
Key Words	Pancreas; RNA; RNA integrity numbers value; RNA isolation; Human biopsy; Organ transplantation; Molecular biology
Core Tip	Obtaining high-quality RNA from human pancreatic tissue remains a persistent challenge in transplant research due to its high endogenous RNase activity and unavoidable pre-analytical ischemia. Using 144 biopsies from four deceased donor pancreata, we systematically evaluated tissue preservation strategies. Our findings demonstrate that both tissue submersion and direct injection of RNAlater yield significantly superior RNA integrity compared with conventional methods. This study provides specific human evidence for a simple and scalable strategy to ensure reliable biobanking and downstream molecular analysis of human pancreatic tissue.
Citation	Pook RR, Hearne A, Botting RA, Tingle SJ, Honkanen-Scott M, Shaw JAM, Ali S, Scott III WE. Improved stabilisation of human pancreas biopsies: Comparative evaluation of RNA preservation methods. World J Biol Chem 2026; In press DOI: https://doi.org/10.4331/wjbc.121018
PDF	121018-in-press.pdf

Received	2026-03-23 07:33
Peer-Review Started	2026-03-23 07:33
First Decision by Editorial Office Director	2026-04-28 10:50
Return for Revision	2026-04-28 10:50
Revised	2026-05-13 12:31
Publication Fee Transferred
Second Decision by Editor	2026-06-23 02:17
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2026-06-23 07:03
Articles in Press	2026-06-23 07:03
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1949-8454 (online)
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Copyright	©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc.
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