Publication Name	World Journal of Biological Chemistry
Manuscript ID	121467
Country	Greece

Category	Medicine, Legal
Manuscript Type	Opinion Review
Article Title	Single-cell deoxyribonucleic acid typing for forensic mixtures and trace evidence: Opportunities, validation requirements, and reporting limits
Manuscript Source	Invited Manuscript
All Author List	Kyriacos Evangelou, Paraskevi Angeli, Andreas Polydorou and Thalia Petropoulou
Funding Agency and Grant Number
Corresponding Author	Kyriacos Evangelou, MD, Department of Minimally Invasive Colon and Rectal Surgery, The Euroclinic Hospital of Athens, 7-9 Athanasiadou & D. Soutsou Street, Athens 11521, Greece. evangeloukyriacos@gmail.com
Key Words	Forensic genetics; Single-cell genomics; Deoxyribonucleic acid mixtures; Laser capture microdissection; Digital cell sorting; Whole-genome amplification; Short tandem repeats; Activity-level propositions; Deoxyribonucleic acid transfer
Core Tip	Single-cell forensic deoxyribonucleic acid (DNA) typing has moved beyond speculation, but present evidence supports targeted use only in validated niches. Its forensic value lies in reducing mixture complexity before computation, preserving cell-of-origin information, and rescuing contributor profiles that bulk analysis may not resolve. Its forensic risk lies in selective cell capture, single-template stochasticity, contamination at near-single-copy sensitivity, and the temptation to over-read cellular context as activity-level proof. The appropriate translational question is therefore not whether single-cell short tandem repeat typing works in principle, but which minimum validation thresholds and reporting limits are necessary before it enters accredited casework.
Citation	Evangelou K, Angeli P, Polydorou A, Petropoulou T. Single-cell deoxyribonucleic acid typing for forensic mixtures and trace evidence: Opportunities, validation requirements, and reporting limits. World J Biol Chem 2026; In press

Received	2026-03-26 03:41
Peer-Review Started	2026-03-26 03:41
First Decision by Editorial Office Director	2026-04-10 08:58
Return for Revision	2026-04-10 08:58
Revised	2026-04-24 08:23
Publication Fee Transferred
Second Decision by Editor	2026-05-09 02:37
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2026-05-09 06:15
Articles in Press	2026-05-09 06:15
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1949-8454 (online)
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