Publication Name	World Journal of Gastrointestinal Oncology
Manuscript ID	124065
DOI	10.4251/wjgo.124065
Country	Spain

Category	Oncology
Manuscript Type	Observational Study
Article Title	Gastric cancer lymph node analysis by one-step nucleic acid amplification assay using individual vs pooling approaches
Manuscript Source	Unsolicited Manuscript
All Author List	Juan Jesús del Río, Marcial García-Rojo, Raquel Escalera Pérez, Mrinalini Honavar, Joana do Santos, Amaro Frutuoso, Marta Santos, João Magalhães, José Barbosa, Patrícia Pontes, Helena Baldaia, Maria Jose Brito, Pedro Fernandes, Ivan Archilla, Sandra Lopez-Prades and Miriam Cuatrecasas
Funding Agency and Grant Number
Corresponding Author	Miriam Cuatrecasas, Pathology Department-Centre de Diagnòstic Biomèdic (CDB), Hospital Clínic. University of Barcelona, Pathology Department-Centre de Diagnòstic Biomèdic (CDB), Hospital Clínic, University of Barcelona, Escala 3, Planta 5., Barcelona 08036, Barcelona, Spain. mcuatrec@clinic.cat
Key Words	Gastric cancer; Lymph node; Staging; One-step nucleic acid amplification; Metastasis; Molecular
Core Tip	Evidence on the accuracy of the one-step nucleic acid amplification (OSNA) assay including individual and pooling approaches for detecting lymph node metastases in gastric cancer (GC) is limited. In this prospective multicenter study, OSNA showed 86% agreement with matched hematoxylin and eosin (H&E) assessments and identified molecular nodal positivity in a higher proportion of patients. The individual and pooling approaches showed similar point estimates for sensitivity and negative predictive value when compared with H&E, while pooling required fewer molecular determinations. These findings support OSNA as a complementary molecular approach for nodal assessment in GC and provide further evidence on the feasibility of pooling.
Citation	del Río JJ, García-Rojo M, Escalera Pérez R, Honavar M, do Santos J, Frutuoso A, Santos M, Magalhães J, Barbosa J, Pontes P, Baldaia H, Brito MJ, Fernandes P, Archilla I, Lopez-Prades S, Cuatrecasas M. Gastric cancer lymph node analysis by one-step nucleic acid amplification assay using individual vs pooling approaches. World J Gastrointest Oncol 2026; In press DOI: https://doi.org/10.4251/wjgo.124065
PDF	124065-in-press.pdf

Received	2026-06-08 02:00
Peer-Review Started	2026-06-08 02:01
First Decision by Editorial Office Director
Return for Revision	2026-08-28 06:05
Revised	2026-09-10 13:02
Publication Fee Transferred
Second Decision by Editor	2026-11-02 02:19
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2026-11-04 10:22
Articles in Press	2026-11-04 10:22
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-5204 (online)
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Copyright	©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc.
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