Publication Name	World Journal of Clinical Oncology
Manuscript ID	126162
DOI	10.5306/wjco.126162
Country	China

Category	Oncology
Manuscript Type	Translational Research
Article Title	Integrated single-cell transcriptomic analysis of brain and lymph node metastases in non small cell lung cancer
Manuscript Source	Unsolicited Manuscript
All Author List	Zhang-Nan Liu, Miao-Miao Li, Yu Ping, Qi-Tai Zhao, Li-Ping Wang and Yi Zhang
Funding Agency and Grant Number
Corresponding Author	Yi Zhang, Chief Physician, Full Professor, MD, PhD, Biotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou 450052, Henan Province, China. yizhang@zzu.edu.cn
Key Words	Non small cell lung cancer; scRNA-seq; T cells; Macrophages; unfolded protein response; Brain metastasis; Lymph node metastasis
Core Tip	Our study offers a panoramic analysis of two distinct metastatic sites within the same disease context. Our main findings are: The distribution of tumor epithelial subpopulations differs across metastatic foci; Abnormal activation of unfolded protein response (UPR) occurs in both metastatic sites; and a dual immunosuppressive microenvironment is characterized. Multivariate Cox regression analysis based on The Cancer Genome Atlas data revealed that, among the tumor epithelial subpopulations, the C14_PBK_Tumor subtype was associated with worse prognosis in lung adenocarcinoma patients. Importantly, multiplex immunohistochemistry was performed to assess macrophage infiltration and the expression of UPR-related proteins. These findings still require further verification through functional experiments and prospective studies.
Citation	Liu ZN, Li MM, Ping Y, Zhao QT, Wang LP, Zhang Y. Integrated single-cell transcriptomic analysis of brain and lymph node metastases in non small cell lung cancer. World J Clin Oncol 2026; In press DOI: https://doi.org/10.5306/wjco.126162
PDF	126162-in-press.pdf

Received	2026-07-28 02:28
Peer-Review Started	2026-08-07 08:49
First Decision by Editorial Office Director
Return for Revision	2026-08-18 08:00
Revised	2026-08-31 10:47
Publication Fee Transferred	2026-09-15 10:05
Second Decision by Editor	2026-10-13 06:01
Second Decision by Editor-in-Chief
Final Decision by Editorial Office Director	2026-10-13 09:55
Articles in Press	2026-10-13 09:55
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	2218-4333 (online)
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