Publication Name	World Journal of Gastroenterology
Manuscript ID	35799
Country	China

Category	Medicine, Research & Experimental
Manuscript Type	Basic Study
Article Title	Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model
Manuscript Source	Unsolicited Manuscript
All Author List	Yi-Chao Chai, Guo-Xin Dang, Hai-Qi He, Jian-Hua Shi, Hong-Ke Zhang, Rui-Tao Zhang, Bo Wang, Liang-Shuo Hu and Yi Lv
Funding Agency and Grant Number	Funding Agency Grant Number the National Natural Science Foundation No. 81470896 the Project of Development and Innovation Team of Ministry of Education No. IRT_16R57
Corresponding Author	Liang-Shuo Hu, MD, Research Associate, Surgeon, Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi’an Jiaotong University, No.277 West Yan-ta Road, Xi’an 710061, Shaanxi, China. huliangshuo1983@hotmail.com
Key Words	Metformin; AMP-activated protein kinase; Cold ischemia injury; Hypothermic machine perfusion; Liver Grafts
Core Tip	Metformin can activate AMP-activated protein kinase pathway that could enhance the activity of endothelial nitric oxide synthase and finally increase the generation of nitric oxide, which plays an important role in the protection of liver sinusoidal endothelial cells. Hence, our study was designed to evaluate protective effect of University of Wisconsin storage solution with metformin for preserving standard and marginal criteria liver grafts of young and aged rats ex vivo by hypothermic machine perfusion (HMP). According to the results, HMP of extracorporeal circulation with metformin can play a significant protective role for liver grafts during cold ischemia, with significant effects especially for the aged-marginal donor.
Citation	Chai YC, Dang GX, He HQ, Shi JH, Zhang HK, Zhang RT, Wang B, Hu LS, Lv Y. Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model. World J Gastroenterol 2017; 23(40): 7221-7231

Received	2017-08-19 01:36
Peer-Review Started	2017-08-19 05:55
To Make the First Decision	2017-08-29 23:50
Return for Revision	2017-09-01 05:46
Revised	2017-09-10 16:17
Second Decision	2017-09-13 01:25
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2017-09-19 20:44
Articles in Press	2017-09-19 20:44
Publication Fee Transferred	2017-09-21 15:00
Edit the Manuscript by Language Editor	2017-10-11 14:02
Typeset the Manuscript	2017-10-23 03:17

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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