Publication Name	World Journal of Gastroenterology
Manuscript ID	43171
Country	Brazil

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Relationship between Fusobacterium nucleatum, inflammatory mediators and microRNAs in colorectal carcinogenesis
Manuscript Source	Invited Manuscript
All Author List	Marcela Alcântara Proença, Joice Matos Biselli, Maysa Succi, Fábio Eduardo Severino, Gustavo Noriz Berardinelli, Alaor Caetano, Rui Manuel Reis, David J Hughes and Ana Elizabete Silva
Funding Agency and Grant Number	Funding Agency Grant Number São Paulo Research Foundation (FAPESP) 2012/15036-8 National Council for Scientific and Technological Development (CNPq) 474.776/2013-1
Corresponding Author	Ana Elizabete Silva, PhD, Adjunct Professor, Teacher, Department of Biology, UNESP São Paulo State University, Rua Cristóvão Colombo 2265, São José do Rio Preto 15054-000, São Paulo, Brazil. ae.silva@ibilce.unesp.br
Key Words	Colorectal cancer; Colorectal adenoma; Fusobacterium nucleatum; Inflammation; Cytokines; MicroRNAs
Core Tip	We examined the influence of Fusobacterium nucleatum (F. nucleatum) in colorectal adenoma (CRA) and colorectal cancer (CRC) on the mRNA expression of inflammatory mediators and the association with microRNA (miRNA) levels, KRAS mutation, and micro­satellite instability (MSI). We suggest that F. nucleatum contributes to CRC development by increasing the expression of inflammatory mediators through a possible miRNA-mediated activation of TLR2/TLR4. The miRNA:mRNA interaction network suggests an upregulation of miR-34a in CRC via a TLR2/TLR4-dependent response to F. nucleatum. KRAS mutations were more frequent in F. nucleatum-infected CRC and were associated with a greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.
Citation	Proença MA, Biselli JM, Succi M, Severino FE, Berardinelli GN, Caetano A, Reis RM, Hughes DJ, Silva AE. Relationship between Fusobacterium nucleatum, inflammatory mediators and microRNAs in colorectal carcinogenesis. World J Gastroenterol 2018; 24(47): 5351-5365

Received	2018-10-30 01:14
Peer-Review Started	2018-10-30 03:14
To Make the First Decision	2018-11-08 06:24
Return for Revision	2018-11-08 07:25
Revised	2018-11-29 19:40
Second Decision	2018-12-13 09:00
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2018-12-13 17:35
Articles in Press	2018-12-13 17:35
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2018-12-20 08:28

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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