Publication Name	World Journal of Gastroenterology
Manuscript ID	49114
Country	China

Category	Infectious Diseases
Manuscript Type	Basic Study
Article Title	Construction of replication-competent hepatitis B virus vector carrying secreted luciferase transgene and new hepatitis B virus replication and expression cell lines
Manuscript Source	Unsolicited Manuscript
All Author List	Jie Ruan, Cai-Yan Ping, Shuo Sun, Xin Cheng, Peng-Yu Han, Yin-Ge Zhang and Dian-Xing Sun
Funding Agency and Grant Number	Funding Agency Grant Number the National Natural Science Foundation of China 81672041 the National Major Science and Technology Special Project for Infectious Diseases of China 2012ZX10004503-012
Corresponding Author	Dian-Xing Sun, PhD, Doctor, Professor, The Liver Disease Center of Chinese People’s Liberation Army, the 980th Hospital of Chinese People’s Liberation Army Joint Logistics Support Force, Shijiazhuang, zhongshanxi street, Shijiazhuang 050082, Hebei Province, China. sundianxing@hotmail.com
Key Words	Hepatitis B virus; Replication-competent hepatitis B virus vector; Secreted luciferase gene; Hepatitis B virus cell line; ;
Core Tip	In this research, we constructed a replication-competent hepatitis B virus (HBV) vector carrying a secreted luciferase transgene and established HBV replication and expression cell lines that could stably secrete secretory NanolucTM Luciferase recombinant viral particles. This vector is a convenient and quantifiable tool for monitoring HBV replication, transcription and expression. It was safe to assume that this new HBV replication system ought to be used in HBV molecular biology research, such as for discovering inhibitors that affect infection, entry, transcription, translation, reverse transcription, and replication.
Citation	Ruan J, Ping CY, Sun S, Cheng X, Han PY, Zhang YG and Sun DX. Construction of replication-competent hepatitis B virus vector carrying secreted luciferase transgene and new hepatitis B virus replication and expression cell lines. World J Gastroenterol 2019; 25(39): 5961-5972

Received	2019-05-20 00:27
Peer-Review Started	2019-05-20 08:51
To Make the First Decision	2019-07-22 03:43
Return for Revision	2019-07-24 02:11
Revised	2019-08-08 08:10
Second Decision	2019-09-12 09:41
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2019-09-13 04:19
Articles in Press	2019-09-13 04:19
Publication Fee Transferred
Edit the Manuscript by Language Editor	2019-09-29 23:14
Typeset the Manuscript	2019-10-18 03:02

ISSN	1007-9327 (print) and 2219-2840 (online)
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