Publication Name	World Journal of Biological Chemistry
Manuscript ID	53536
Country	Spain

Category	Microbiology
Manuscript Type	Review
Article Title	Why the discovery of adherent-invasive Escherichia coli molecular markers is so challenging?
Manuscript Source	Invited Manuscript
All Author List	Carla Camprubí-Font and Margarita Martinez-Medina
Funding Agency and Grant Number	Funding Agency Grant Number Spanish Ministry of Economy and Competitiveness SAF2017-82261-P
Corresponding Author	Margarita Martinez-Medina, PhD, Associate Professor, Laboratory of Molecular Microbiology, Department of Biology, Universitat de Girona, C/Maria Aurèlia Capmany, 40, Girona 17003, Spain. marga.martinez@udg.edu
Key Words	Crohn’s disease; Adherent-invasive Escherichia coli; Molecular markers; Genetics; Inflammatory bowel disease; Signature sequences
Core Tip	In this review, we thoroughly review the approaches for deciphering adherent-invasive Escherichia coli (AIEC) genetics. The characteristics of putative AIEC molecular markers that could assist in AIEC identification are described. We then discuss several aspects that could explain the difficulty behind the discovery of suitable biomarkers and highlight the importance of standardizing AIEC protocols in order to increase the probability of finding these biomarkers. Finally, we point out new approaches for looking for signature sequences that need to take into account the AIEC phylogenetic origin and strain virulence under particular experimental conditions.
Citation	Camprubí-Font C, Martinez-Medina M. Why the discovery of adherent-invasive Escherichia coli molecular markers is so challenging? World J Biol Chem 2020; In press

Received	2019-12-23 14:38
Peer-Review Started	2019-12-23 14:39
To Make the First Decision	2020-02-26 11:18
Return for Revision	2020-02-27 11:17
Revised	2020-03-18 12:03
Second Decision	2020-03-30 12:36
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-03-31 19:11
Articles in Press	2020-03-31 19:11
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2020-04-07 06:15

ISSN	1949-8454 (online)
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