Publication Name	World Journal of Stem Cells
Manuscript ID	55679
Country	Japan

Category	Cell Biology
Manuscript Type	Review
Article Title	Practical choice to robust and efficient differentiation of human pluripotent stem cells
Manuscript Source	Invited Manuscript
All Author List	Mei Fang, Li-Ping Liu, Hang Zhou, Yu-Mei Li and Yun-Wen Zheng
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 81770621 Ministry of Education, Culture, Sports, Science, and Technology of Japan, KAKENHI 18H02866 Natural Science Foundation of Jiangsu Province BK20180281
Corresponding Author	Yun-Wen Zheng, PhD, Associate Professor, Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, University of Tsukuba Faculty of Medicine, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8575, Japan. ywzheng@md.tsukuba.ac.jp
Key Words	Human pluripotent stem cells; Three dimensional; Embryoid body; Differentiation; Efficient; Three germ layers
Core Tip	It is essential for regenerative medicine research to propose a practical way of efficient differentiation of pluripotent stem cells. After considering the advantages and limitations of current approaches, we summarized the ideal conditions and systems. We also provided potential choices for differentiating human pluripotent stem cells efficiently and robustly into target cells and tissues in different germ layers.
Citation	Fang M, Liu LP, Zhou H, Li YM, Zheng YW. Practical choice to robust and efficient differentiation of human pluripotent stem cells. World J Stem Cells 2020; 12(8): 752-760

Received	2020-03-28 15:24
Peer-Review Started	2020-03-28 15:26
To Make the First Decision
Return for Revision	2020-04-22 16:13
Revised	2020-04-30 04:28
Second Decision	2020-06-30 09:16
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-07-01 22:48
Articles in Press	2020-07-01 22:48
Publication Fee Transferred
Edit the Manuscript by Language Editor	2020-07-10 21:54
Typeset the Manuscript	2020-08-07 04:27

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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