Publication Name	World Journal of Gastroenterology
Manuscript ID	59445
Country	United States

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Effect of acetyl-L-carnitine on hypersensitivity in acute recurrent caerulein-induced pancreatitis and microglial activation along the brain’s pain circuitry
Manuscript Source	Unsolicited Manuscript
All Author List	Sabrina L McIlwrath, Marlene E Starr, Abigail E High, Hiroshi Saito and Karin N Westlund
Funding Agency and Grant Number	Funding Agency Grant Number United States Department of Veterans Affairs, VA Merit Grant BX002695 United States National Institute of Health R01AG055359 United States National Institute of Health R01GM126181 United States National Institute of Health R01NS39041-15
Corresponding Author	Sabrina L McIlwrath, PhD, Senior Scientist, Research Service, New Mexico Veterans Affairs Healthcare System, 1501 San Pedro SE, Albuquerque, NM 87108, United States. sabrina.mcilwrath@va.gov
Key Words	Acute recurrent pancreatitis; Neuropathic pain; Mechanical hypersensitivity; Heat hypersensitivity; Anxiety-like behavior; Ionized calcium-binding adaptor molecule 1
Core Tip	The caerulein- (CAE) induced pancreatitis model requiring 6 wk of repeated injections is a model of recurrent acute bouts of pancreatitis that causes pancreatic tissue damage and fibrosis. Control repeated i.p. saline injections alone caused abdominal wall injury and hindpaw secondary mechanical hypersensitivity. Treatment with acetyl-L-carnitine significantly attenuated CAE-induced hypersensitivity without alleviating pancreatic histological disruption. Mice with CAE-induced pancreatitis with secondary mechanical and heat hypersensitivity had elevated plus maze anxiety-like behavior. Post-mortem analysis revealed microglial morphology changes indicative of activation in amygdala, hippocampus, hypothalamus, and thalamus, but not in primary somatosensory cortex. These data suggest that activated microglia in these brain regions contribute to chronic hypersensitivity and anxiety-like behaviors in mice with CAE-induced pancreatitis.
Citation	McIlwrath SL, Starr ME, High AE, Saito H, Westlund KN. Effect of acetyl-L-carnitine on hypersensitivity in acute recurrent caerulein-induced pancreatitis and microglial activation along the brain’s pain circuitry. World J Gastroenterol 2021; 27(9): 794-814

Received	2020-09-11 22:14
Peer-Review Started	2020-09-11 22:14
To Make the First Decision
Return for Revision	2020-11-25 20:24
Revised	2020-12-08 23:31
Second Decision	2021-01-15 13:40
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2021-01-15 17:38
Articles in Press	2021-01-15 17:38
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2021-03-02 11:36

ISSN	1007-9327 (print) and 2219-2840 (online)
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