Publication Name	World Journal of Stem Cells
Manuscript ID	99326
Country	China

Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures
Manuscript Source	Unsolicited Manuscript
All Author List	He-Ao Zhang, Bo-Yu Zhang and Hong-Bo Tang
Funding Agency and Grant Number
Corresponding Author	Hong-Bo Tang, Professor, Department of Plastic and Cosmetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Avenue, Wuhan 430030, Hubei Province, China. tthhbb@sohu.com
Key Words	Fracture; Adipose tissue-derived stem cells; Osteogenic differentiation; Three-dimensional cell culture; N-cadherin
Core Tip	The macrophages in three-dimensional (3D) culture inhibited osteogenic differentiation of adipose tissue-derived stem cells (ADSCs), contrary to the effect in 2D culture. The 3D culture environment might inhibit the Wnt/β-catenin signaling pathway by increasing N-cadherin expression, and consequently, inhibiting osteogenic differentiation of ADSCs. This study offers a refined protocol for the exploration of ADSCs osteogenesis and provides novel ideas for exploring 3D osteogenesis in ADSCs, fracture repair, and other bone trauma repair.
Citation	<p>Zhang HA, Zhang BY, Tang HB. Effects of macrophages on the osteogenic differentiation of adipose tissue-derived stem cells in two-dimensional and three-dimensional cocultures. <i>World J Stem Cells</i> 2025; 17(2): 99326</p>

Received	2024-07-19 15:14
Peer-Review Started	2024-07-19 15:14
To Make the First Decision
Return for Revision	2024-10-30 09:50
Revised	2024-11-24 11:17
Second Decision	2025-01-11 01:49
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-01-23 09:06
Articles in Press	2025-01-23 09:06
Publication Fee Transferred	2024-11-30 03:13
Edit the Manuscript by Language Editor
Typeset the Manuscript	2025-02-09 13:21

ISSN	1948-0210 (online)
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