Publication Name	World Journal of Gastroenterology
Manuscript ID	36386
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Cell culture-adaptive mutations in hepatitis C virus promote viral production by enhancing viral replication and release
Manuscript Source	Unsolicited Manuscript
All Author List	Qi Wang, Yue Li, Shun-Ai Liu, Wen Xie and Jun Cheng
Funding Agency and Grant Number	Funding Agency Grant Number Beijing Municipal Administration of Hospitals’ Youth Program QML20161801 Beijing Municipal Administration of Hospital Programs DFL2015170 Beijing Municipal Administration of Hospital Programs ZYLX201402 National Natural Science Foundation of China 81500439
Corresponding Author	Jun Cheng, MD, PhD, Professor, Center of Liver Diseases, Beijing Ditan Hospital, Capital Medical University, No. 8 East Jingshun Street, Chaoyang District, Beijing 100015, China. chengj0817@ccmu.edu.cn
Key Words	Hepatitis C virus; JFH1; Adaptive mutation; RNA replication; Virion release; Lipid droplet localization
Core Tip	In this study, we explored hepatitis C virus (HCV) adaptive mutations or combinations thereof responsible for enhanced viral production and inves­tigated the underlying mechanisms. We generated infectious HCV particles with a robust titer of 1.61 × 106 focus-forming units (FFUs)/mL, and confirmed that the adaptive mutations could enhance viral replica­tion and release. The results were established at the levels of infectious particle titers, HCV RNA, protein expression, virus release, lipid droplet, and NS5A co-localization, and further the ratio between p56 and p58 phosphoforms of NS5A.
Citation	Wang Q, Li Y, Liu SA, Xie W, Cheng J. Cell culture-adaptive mutations in hepatitis C virus promote viral production by enhancing viral replication and release. World J Gastroenterol 2018; 24(12): 1299-1311

Received	2017-12-01 03:40
Peer-Review Started	2017-12-01 14:19
To Make the First Decision	2017-12-20 07:42
Return for Revision	2017-12-21 12:34
Revised	2018-01-04 03:52
Second Decision	2018-01-18 09:48
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2018-01-18 15:09
Articles in Press	2018-01-18 15:09
Publication Fee Transferred
Edit the Manuscript by Language Editor	2018-02-08 07:38
Typeset the Manuscript	2018-03-15 09:14

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com