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Publication Name World Journal of Stem Cells
Manuscript ID 42952
Country of Manuscript Source Taiwan
Specialty Physiology
Manuscript Type Basic Study
Article Title Functional and molecular mechanism of intracellular pH regulation in human inducible pluripotent stem cells
Manuscript Source Invited Manuscript
All Author List Shih-Chi Chao, Gwo-Jang Wu, Shu-Fu Huang, Nian-Tzy Dai, Hsu-Kai Huang, Shiao-Pieng Lee, Mei-Fang Chou, Yi-Ting Tsai and Shih-Hurng Loh
Funding Agency and Grant Number
Funding Agency Grant Number
Ministry of Science and Technology Grants of Taiwan MOST 106-2320-B-016-003-MY2 (to Loh SH)
Ministry of Science and Technology Grants of Taiwan MOST 106-2314-B-016 -037-MY3 (to Tsai YT)
National Defense Medical Center Grants of Taiwan MAB-106-033 (to Loh SH)
National Defense Medical Center Grants of Taiwan MAB-105-043 (to Dai NZ)
National Defense Medical Center Grants of Taiwan MAB-106-034 (to Dai NZ)
Teh-Tzer Study Group for Human Medical Research Foundation of Taiwan A1061037 (to Loh SH)
Teh-Tzer Study Group for Human Medical Research Foundation of Taiwan A1061054 (to Loh SH)
Correspondence To Shih-Hurng Loh, DPhil, Director, Full Professor, Chairman and Director, Department of Pharmacology, and Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical Center, No. 161, Sec. 6, Minquan E. Rd., Taipei 11490, Taiwan. shloh@ndmctsgh.edu.tw
Keywords Human induced pluripotent stem cells; Intracellular pH; Na+/H+ exchanger; Na+/HCO3- cotransporter; Cl-/OH- exchanger; Cl-/HCO3- exchanger; v-ATPase; Intracellular buffering power; BCECF; Microspectrofluorimetry
Core Tip For the first time, we established a model of the intracellular pH (pHi) regulation mechanism in human induced pluripotent stem cells (hiPSCs). The steady-state pHi value of hiPSCs was 7.50-7.68, which greater than that of normal adult cells. The Na+-H+ exchanger, the Na+-HCO3- cotransporter and vacuolar-ATPase were the main acid extruders, while the Cl--HCO3- anion exchanger and the Cl--OH- exchanger were the main acid loaders. Moreover, the pHi and acid-extruding mechanism were decreased during the loss of pluripotency in hiPSCs. pHi regulators represent an attractive target for differentiation efficiency or culture quality.
Citation Chao SC, Wu GJ, Huang SF, Dai NT, Huang HK, Chou MF, Tsai YT, Lee SP, Loh SH. Functional and molecular mechanism of intracellular pH regulation in human inducible pluripotent stem cells. World J Stem Cells 2018; In press
Received
2018-10-17 03:02
Peer-Review Started
2018-10-17 08:54
To Make the First Decision
2018-10-29 02:45
Return for Revision
2018-11-05 09:19
Revised
2018-11-14 09:12
Second Decision
2018-12-03 00:51
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2018-12-05 05:21
Articles in Press
2018-12-05 05:21
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
ISSN 1948-0210 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7901 Stoneridge Drive, Suite 501, Pleasanton, CA 94588, USA
Website http://www.wjgnet.com
Assistant Editor Xiang Li
Science Editor Jin-Lei Wang
Reviewer Code 03370303, and 03478635
Editorial Director Fang-Fang Ji
Journal Chief Editor
Company Chief Editor Li Ma
Language Editor
Electronic Editor
Responsible Electronic Editor
Responsible Science Editor
Proofing Editorial Director Jin-Lei Wang
Proofing Editor-in-Chief Lian-Sheng Ma
Revised Manuscript 42952-Review.docx
Peer-review Report 42952-Peer-review(s).pdf
Answering Reviewers 42952-Answering reviewers.pdf
Scientific Misconduct Check 42952-Scientific misconduct check.pdf
Scientific Editor Work List 42952-Scientific editor work list.pdf
Audio Core Tip 42952-Audio core tip.mp3
Biostatistics Review Certificate 42952-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 42952-Conflict-of-interest statement.pdf
Copyright License Agreement 42952-Copyright assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 42952-Grant application form(s).pdf
Institutional Review Board Approval Form or Document 42952-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 42952-Language certificate.pdf