Publication Name	World Journal of Gastroenterology
Manuscript ID	46761
Country	China

Category	Radiology, Nuclear Medicine & Medical Imaging
Manuscript Type	Basic Study
Article Title	Development and in vitro study of bi-specific magnetic resonance imaging molecular probe for hepatocellular carcinoma
Manuscript Source	Unsolicited Manuscript
All Author List	Xiao-Hong Ma, Shuang Wang, Si-Yun Liu, Kun Chen, Zhi-Yuan Wu, Deng-Feng Li, Yong-Tao Mi, Long-Bin Hu, Zhong-Wei Chen and Xin-Ming Zhao
Funding Agency and Grant Number	Funding Agency Grant Number CAMS Innovation Fund for Medical Sciences 2016-I2M-1-001 PUMC Youth Fund 2017320010 Chinese Academy of Medical Sciences Research Fund ZZ2016B01 Beijing HopeRun Special Fund of Cancer Foundation of China LC2016B15
Corresponding Author	Xin-Ming Zhao, BM BCh, Chairman, Chief Doctor, Professor, Department of Diagnostic Radiology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.17, Panjiayuan Nanli, Chaoyang District, Beijing 100021, China. zhaoxinming@cicams.ac.cn
Key Words	Hepatocellular carcinoma; Molecular imaging; Magnetic resonance imaging; Ultra-small superparamagnetic iron nanoparticles; Alpha-fetoprotein; Glypican-3
Core Tip	The singly targeting of existing hepatocellular carcinoma-targeted magnetic resonance imaging (HCC-targeted MRI) probes may weaken the detection efficiency as biomarker associated tumor heterogeneity. Here, double-antibody-labeled ultra-small superparamagnetic iron nanoparticles (USPIO) were synthesized to simultaneously target HCC markers of alpha- fetoprotein (AFP) and Glypican-3 (GPC3) antigens in Hepa1-6/GPC3 cells. Such probes showed higher cancer cell labeling efficiency than singly- or non-targeted USPIO probes by Prussian blue staining and in vitro MRI, indicating enhanced specificity and sensitivity of micro hepatocellular carcinoma (MHCC) MRI diagnosis. Meanwhile, USPIO with small core (~5 nm) and hydrodynamic size (~60 nm) after antibodies labelling may undergo slow phagocytosis, which could enhance liver tumor MRI contrast in the animal or clinical trial study.
Citation	Ma XH, Wang S, Liu SY, Chen K, Wu ZY, Li DF, Mi YT, Hu LB, Chen ZW, Zhao XM. Development and in vitro study of bi-specific magnetic resonance imaging molecular probe for hepatocellular carcinoma. World J Gastroenterol 2019; 25(24): 3030-3043

Received	2019-02-25 00:49
Peer-Review Started	2019-02-25 06:36
To Make the First Decision	2019-03-20 06:29
Return for Revision	2019-03-20 08:04
Revised	2019-04-03 08:18
Second Decision	2019-05-17 08:54
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2019-05-18 12:49
Articles in Press	2019-05-18 12:49
Publication Fee Transferred
Edit the Manuscript by Language Editor	2019-05-29 01:50
Typeset the Manuscript	2019-06-28 08:58

ISSN	1007-9327 (print) and 2219-2840 (online)
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