Publication Name	World Journal of Gastroenterology
Manuscript ID	47640
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Retrospective Cohort Study
Article Title	Liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients
Manuscript Source	Unsolicited Manuscript
All Author List	Wen-Ying Xia, Li Gao, Er-Hei Dai, Dan Chen, Er-Fu Xie, Li Yang, Shi-Chang Zhang, Bing-Feng Zhang, Jian Xu and Shi-Yang Pan
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 81672100 National Natural Science Foundation of China 81371894 Key Laboratory for Laboratory Medicine of Jiangsu Province of China ZDXKB2016005 Jiangsu Provincial Commission of Health and Family Planning H201609
Corresponding Author	Shi-Yang Pan, MD, Professor, Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Guangzhou Road No. 300, Nanjing 210029, Jiangsu Province, China. sypan@njmu.edu.cn
Key Words	Liquid biopsy; plasma DNA; Hepatitis B; Alanine aminotransferase; Duplex real-time quantitative polymerase chain reaction;
Core Tip	Our study quantified cell free DNA combined with other serum biomarker as a liquid biopsy-based method to assess liver injury in hepatitis B patients. A cohort of 663 subjects including 313 hepatitis B patients and 350 healthy controls were enrolled. The ultrasound-guided liver biopsies followed by histopathological assessments were performed for the 263 chronic hepatitis B patients to determine the degree of liver injury. Cell-free DNA was quantified using a novel duplex real-time polymerase chain reaction assay. Our results demonstrated that the combination of plasma DNA, serum alanine aminotransferase and bilirubin could be a candidate liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients.
Citation	Xia WY, Gao L, Dai EH, Chen D, Xie EF, Yang L, Zhang SC, Zhang BF, Xu J, Pan SY. Liquid biopsy for non-invasive assessment of liver injury in hepatitis B patients. World J Gastroenterol 2019; 25(29): 3985-3995

Received	2019-03-26 02:20
Peer-Review Started	2019-03-26 04:55
To Make the First Decision	2019-05-27 00:35
Return for Revision	2019-05-31 01:50
Revised	2019-06-13 15:31
Second Decision	2019-07-05 10:26
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2019-07-05 23:48
Articles in Press	2019-07-05 23:48
Publication Fee Transferred
Edit the Manuscript by Language Editor	2019-07-15 03:35
Typeset the Manuscript	2019-08-05 08:32

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com