Publication Name	World Journal of Gastroenterology
Manuscript ID	50819
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Characterizing the composition of intestinal microflora by 16S rRNA gene sequencing
Manuscript Source	Unsolicited Manuscript
All Author List	Wen-Jia Wang, You-Lian Zhou, Jie He, Zhi-Qiang Feng, Long Zhang, Xiao-Bo Lai, Jun-Xiao Zhou and Hong Wang
Funding Agency and Grant Number	Funding Agency Grant Number Guangdong Provincial Department of Science and Technology 2014A020212568 National Key Clinical Specialized Special Funds Programs of China 2013544
Corresponding Author	Hong Wang, MD, PhD, Chief Physician, Department of Gastroenterology,Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, South China University of Technology, No. 1 Panfu road, Yuexiu district, Guangzhou 510030, Guangdong Province, China. wong.hong@163.com
Key Words	16S rDNA; Intestinal microflora; Advanced colorectal adenoma; Colorectal cancer; Biomarkers;
Core Tip	Colorectal adenoma (CRA), as the most important pre-cancerous lesion of colorectal cancer, has a close relationship with the intestinal microflora. But the mechanism is not clear. Early detection of CRA can effectively reduce the morbidity and mortality of colorectal cancer. This paper analyzes the composition and diversity of intestinal microflora in patients with CRA through 16s rDNA gene sequencing. It was proved that the diversity of intestinal microflora in patients with CRA decreased, and the increase in the number of Halomonadaceae and Shewanella algae may be biomarkers of CRA, which provides a basis for future research.
Citation	Wang WJ, Zhou YL, He J, Feng ZQ, Zhang L, Lai XB, Zhou JX, Wang H. Characterizing the composition of intestinal microflora by 16S rRNA gene sequencing. World J Gastroenterol 2020; 26(6): 614-626

Received	2019-08-13 09:14
Peer-Review Started	2019-08-13 09:14
To Make the First Decision	2019-10-14 08:55
Return for Revision	2019-10-17 02:43
Revised	2019-12-25 01:31
Second Decision	2020-01-08 09:34
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-01-11 14:20
Articles in Press	2020-01-11 14:20
Publication Fee Transferred
Edit the Manuscript by Language Editor	2020-01-16 21:24
Typeset the Manuscript	2020-02-10 09:44

ISSN	1007-9327 (print) and 2219-2840 (online)
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