Publication Name	World Journal of Hepatology
Manuscript ID	53546
Country	United Kingdom

Category	Pathology
Manuscript Type	Basic Study
Article Title	High omega arachidonic acid/docosahexaenoic acid ratio induces mitochondrial dysfunction and altered lipid metabolism in human hepatoma cells
Manuscript Source	Unsolicited Manuscript
All Author List	Reem Ghazali, Kosha J Mehta, SW Annie Bligh, Ihab Tewfik, Dahn Clemens and Vinood B Patel
Funding Agency and Grant Number
Corresponding Author	Vinood B Patel, FRSC, PhD, Reader (Associate Professor), School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, United Kingdom. v.b.patel@westminster.ac.uk
Key Words	Non alcoholic fatty liver disease; Lipogenesis; Omega fatty acids; Mitochondrial dysfunction; Reactive oxygen species; Oxidative stress
Core Tip	A high ratio of omega 6:3 fatty acids in the diet has been implicated in the develop-ment of non alcoholic fatty liver disease, a growing epidemic of major concern. The cellular pathology induced by such high ratios remains unknown. Here, we ob-served that in human hepatoma HepG2 (VL-17A) cells, high omega-6:omega-3 ratio reduced mitochondrial activity, increased triglyceride accumulation, elevated reac-tive oxygen species levels and interrupted several mitochondrial functions. Moreo-ver, the increased expression of stearoyl-CoA desaturase, decreased expression of peroxisome proliferator-activated receptor alpha and elevation in cannabinoid recep-tor-1 expression collectively lead to lipogenesis and lipotoxicity, which are key fea-tures of non alcoholic fatty liver disease development.
Citation	Ghazali R, Mehta KJ, Bligh SA, Tewfik I, Dahn Clemens D, Patel VB. High omega arachidonic acid/docosahexaenoic acid ratio induces mitochondrial dysfunction and altered lipid metabolism in human hepatoma cells. World J Hepatol 2020; 12(3): 84-98

Received	2019-12-24 07:18
Peer-Review Started	2019-12-23 16:49
To Make the First Decision	2019-12-24 07:35
Return for Revision	2019-12-24 07:35
Revised	2019-12-24 07:52
Second Decision	2020-01-11 10:38
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-01-15 00:45
Articles in Press	2020-01-15 00:45
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2020-03-18 03:30

ISSN	1948-5182 (online)
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