Publication Name	World Journal of Stem Cells
Manuscript ID	55683
Country	Germany

Category	Cardiac & Cardiovascular Systems
Manuscript Type	Basic Study
Article Title	In vivo cardiac pacemaker function of differentiated human mesenchymal stem cells from adipose tissue transplanted into porcine hearts
Manuscript Source	Invited Manuscript
All Author List	Fabrice F Darche, Rasmus Rivinius, Ann-Kathrin Rahm, Eva Köllensperger, Uwe Leimer, Günter Germann, Miriam Reiss, Michael Koenen, Hugo A Katus, Dierk Thomas and Patrick A Schweizer
Funding Agency and Grant Number	Funding Agency Grant Number Max-Planck-Society (TANDEM project to Koenen M and Schweizer PA) Ministry of Science, Research and the Arts Baden-Wuerttemberg (Sonderlinie Medizin to Thomas D) German Heart Foundation (Kaltenbach scholarship to Darche FF) German Cardiac Society (Otto-Hess scholarship to Rahm AK) Heidelberg Medical Faculty (Physician Scientist-Programm to Darche FF, Rivinius R and Rahm AK) German Cardiac Society (Research scholarship to Rivinius R) the German Society of Internal Medicine (Clinician-Scientist-Program to Rahm AK) the German Centre for Cardiovascular Research (DZHK)
Corresponding Author	Patrick A Schweizer, MD, Associate Professor, Senior Scientist, Department of Cardiology, University of Heidelberg, INF 410, Heidelberg D-69120, Germany. patrick.schweizer@med.uni-heidelberg.de
Key Words	Biological cardiac pacemaker; Mesenchymal stem cells; Differentiation; Animal model; Cell transplantation; Stem cell therapy
Core Tip	Differentiated human mesenchymal stem cells from adipose tissue (dhaMSC) transplanted into the myocardium of domestic pigs are able to generate a biological pacemaker system in vivo. Electrocardiogram recordings and immunohistochemical analyses of pigs with transplanted dhaMSC indicated successful biological pacemaker generation. Preconditioning of cells was achieved by in vitro treatment of human mesenchymal stem cells in differentiation culture medium. Due to their reduced immunoreactivity, xenogeneic transplantation of human MSC into pig hearts was feasible without immunosuppression. dhaMSC maintained their immunoprivileged status over time.
Citation	Darche FF, Rivinius R, Rahm AK, Köllensperger E, Leimer U, Germann G, Reiss M, Koenen M, Katus HA, Thomas D, Schweizer PA. In vivo cardiac pacemaker function of differentiated human mesenchymal stem cells from adipose tissue transplanted into porcine hearts. World J Stem Cells 2020; 12(10): 1133-1151

Received	2020-03-28 17:02
Peer-Review Started	2020-03-28 17:02
To Make the First Decision
Return for Revision	2020-04-22 16:11
Revised	2020-05-03 20:13
Second Decision	2020-08-24 12:45
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-08-25 05:29
Articles in Press	2020-08-25 05:29
Publication Fee Transferred
Edit the Manuscript by Language Editor	2020-09-10 05:59
Typeset the Manuscript	2020-10-18 09:16

ISSN	1948-0210 (online)
Open Access	Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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