Publication Name	World Journal of Gastroenterology
Manuscript ID	57487
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Identification of differentially expressed genes in ulcerative colitis and verification in a colitis mouse model by bioinformatics analyses
Manuscript Source	Unsolicited Manuscript
All Author List	Lei Shi, Xiao Han, Jun-Xiang Li, Yu-Ting Liao, Fu-Shun Kou, Zhi-Bin Wang, Rui Shi, Xing-Jie Zhao, Zhong-Mei Sun and Yu Hao
Funding Agency and Grant Number	Funding Agency Grant Number Chinese Medicine Inheritance and Innovation "One Hundred Million" Talent Project Qihuang Scholar (Jun-Xiang Li) The National Key R&D Program of China during the 13th Five-Year Plan Period 2018YFC1705405 The 66th China Postdoctoral Science Foundation 2019M660575
Corresponding Author	Yu Hao, PhD, Professor, Department of Immunology and Microbiology, School of Life Sciences, Beijing University of Chinese Medicine, No. 11 North Third Ring East Road, Chaoyang District, Beijing 100029, China. yuhao64@sina.com
Key Words	Ulcerative colitis; Bioinformatics analysis; C-X-C motif chemokine ligand 13; Neuropeptide Y receptor Y1; C-X-C motif chemokine receptor 2; Colitis model mice
Core Tip	Two microarray datasets from the National Center for Biotechnology Information-Gene Expression Omnibus were used, and differentially expressed genes (DEGs) were analyzed using GEO2R and Venn diagrams. 177 DEGs, 118 upregulated and 59 downregulated, were initially identified from the GEO2R analysis and were found to predominantly participate in inflammation-related pathways. Seventeen core genes were upregulated and 1 was downregulated in the 1st cluster according to protein-protein interaction and Molecular Complex Detection analyses in Cytoscape. These genes were markedly enriched in the cytokine-cytokine receptor interaction and chemokine signaling pathways. Top 3 core genes [C-X-C motif chemokine ligand 13, neuropeptide Y receptor Y1, C-X-C motif chemokine receptor 2 (CXCR2)] in the 1st cluster were verified in dextran sulfate sodium-induced colitis model mice by real-time polymerase chain reaction and showed an increased expression compared with that in the blank, but only CXCR2 was statistically different. CXCR2 may represent a new biomarker to determine the degree of inflammation or a treatment target in ulcerative colitis (UC). In the future, the combination of CXCR2 with other biomarkers will potentially improve the ability to diagnose and dynamically monitor UC.
Citation	Shi L, Han X, Li JX, Liao YT, Kou FS, Wang ZB, Shi R, Zhao XJ, Sun ZM, Hao Y. Identification of differentially expressed genes in ulcerative colitis and verification in a colitis mouse model by bioinformatics analyses. World J Gastroenterol 2020; 26(39): 5983-5996

Received	2020-06-10 15:17
Peer-Review Started	2020-06-10 15:18
To Make the First Decision
Return for Revision	2020-08-22 22:05
Revised	2020-08-30 09:48
Second Decision	2020-09-15 12:11
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-09-15 23:36
Articles in Press	2020-09-15 23:36
Publication Fee Transferred
Edit the Manuscript by Language Editor	2020-09-20 15:01
Typeset the Manuscript	2020-10-18 08:57

ISSN	1007-9327 (print) and 2219-2840 (online)
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