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Publication Name World Journal of Gastroenterology
Manuscript ID 57487
Country/Territory China
Category Gastroenterology and Hepatology
Manuscript Type Basic Study
Article Title Identification of differentially expressed genes in ulcerative colitis and verification in a colitis mouse model by bioinformatics analyses
Manuscript Source Unsolicited Manuscript
All Author List Lei Shi, Xiao Han, Jun-Xiang Li, Yu-Ting Liao, Fu-Shun Kou, Zhi-Bin Wang, Rui Shi, Xing-Jie Zhao, Zhong-Mei Sun and Yu Hao
Funding Agency and Grant Number
Funding Agency Grant Number
Chinese Medicine Inheritance and Innovation "One Hundred Million" Talent Project Qihuang Scholar (Jun-Xiang Li)
The National Key R&D Program of China during the 13th Five-Year Plan Period 2018YFC1705405
The 66th China Postdoctoral Science Foundation 2019M660575
Corresponding author Yu Hao, PhD, Professor, Department of Immunology and Microbiology, School of Life Sciences, Beijing University of Chinese Medicine, No. 11 North Third Ring East Road, Chaoyang District, Beijing 100029, China. yuhao64@sina.com
Key Words Ulcerative colitis; Bioinformatics analysis; C-X-C motif chemokine ligand 13; Neuropeptide Y receptor Y1; C-X-C motif chemokine receptor 2; Colitis model mice
Core Tip Two microarray datasets from the National Center for Biotechnology Information-Gene Expression Omnibus were used, and differentially expressed genes (DEGs) were analyzed using GEO2R and Venn diagrams. 177 DEGs, 118 upregulated and 59 downregulated, were initially identified from the GEO2R analysis and were found to predominantly participate in inflammation-related pathways. Seventeen core genes were upregulated and 1 was downregulated in the 1st cluster according to protein-protein interaction and Molecular Complex Detection analyses in Cytoscape. These genes were markedly enriched in the cytokine-cytokine receptor interaction and chemokine signaling pathways. Top 3 core genes [C-X-C motif chemokine ligand 13, neuropeptide Y receptor Y1, C-X-C motif chemokine receptor 2 (CXCR2)] in the 1st cluster were verified in dextran sulfate sodium-induced colitis model mice by real-time polymerase chain reaction and showed an increased expression compared with that in the blank, but only CXCR2 was statistically different. CXCR2 may represent a new biomarker to determine the degree of inflammation or a treatment target in ulcerative colitis (UC). In the future, the combination of CXCR2 with other biomarkers will potentially improve the ability to diagnose and dynamically monitor UC.
Citation Shi L, Han X, Li JX, Liao YT, Kou FS, Wang ZB, Shi R, Zhao XJ, Sun ZM, Hao Y. Identification of differentially expressed genes in ulcerative colitis and verification in a colitis mouse model by bioinformatics analyses. World J Gastroenterol 2020; In press
Received
2020-06-10 15:17
Peer-Review Started
2020-06-10 15:18
To Make the First Decision
Return for Revision
2020-08-22 22:05
Revised
2020-08-30 09:48
Second Decision
2020-09-15 12:11
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2020-09-15 23:36
Articles in Press
2020-09-15 23:36
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
ISSN 1007-9327 (print) and 2219-2840 (online)
Open Access This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Assistant Editor Assistant Editor
Science Editor Pan Huang
Reviewer Code 01219902, and 02545023
Editorial Director
Journal Chief Editor
Company Chief Editor Lian-Sheng Ma
Language Editor
Production Editor
Responsible Production Editor
Responsible Science Editor
Proofing Editorial Director Jin-Lei Wang
Proofing Editor-in-Chief Lian-Sheng Ma
Manuscript File 57487-Review.docx
Answering Reviewers 57487-Answering reviewers.pdf
Audio Core Tip 57487-Audio core tip.mp3
Biostatistics Review Certificate 57487-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 57487-Conflict-of-interest statement.pdf
Copyright License Agreement 57487-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 57487-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document 57487-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document 57487-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 57487-Language certificate.pdf
Peer-review Report 57487-Peer-review(s).pdf
Scientific Misconduct Check 57487-Bing-Yan JP-1.png
Scientific Misconduct Check 57487-Scientific misconduct check.pdf
Scientific Editor Work List 57487-Scientific editor work list.pdf