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Articles in Press
9/15/2020 11:30:33 PM | Browse: 120 | Download: 396
| Category |
Biochemistry & Molecular Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Mahim Khan, Waqar Rauf, Fazal-e- Habib, Moazur Rahman and Mazhar Iqbal |
| Funding Agency and Grant Number |
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| Corresponding Author |
Mazhar Iqbal, PhD, Professor, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Jhang Road,, Faisalabad 38000, Punjab, Pakistan. hamzamgondal@gmail.com |
| Key Words |
Hepatitis C virus genotype 3a; Non-structural protein 3 protease; Fluorescence resonance energy transfer assay; Citrus extract; Mass spectrometry; Hesperidin |
| Core Tip |
The manuscript describes the screening of active metabolites in citrus fruit extracts against hepatitis c virus genotype3a non-structural protein 3 (HCV-G3a NS3) protease. In this study, conditions have been optimized to get highly purified and functionally active protein HCV NS3. Further, fluorescence resonance energy transfer assay was used to screen the citrus extracts against NS3 protease. By using liquid chromatography coupled with tandem mass spectrometry/ mass spectrometry analysis and bioinformatics modelling approaches, the observed activity of citrus extracts against HCV genotype3a NS3 protease was ascribed to hesperidin. Fluorescence resonance energy transfer assay confirmed the inhibitory potential of hesperidin against NS4A-NS3 protease domain with an IC50 value of 23.32 µM. |
| Citation |
Khan M, Rauf W, Habib F, Rahman M, Iqbal M. Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry. World J Hepatol 2020; 12(11): 976-992 |
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Received |
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2020-07-27 13:44 |
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Peer-Review Started |
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2020-07-27 13:44 |
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To Make the First Decision |
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Return for Revision |
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2020-08-22 18:43 |
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Revised |
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2020-09-03 11:38 |
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Second Decision |
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2020-09-15 12:36 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Company Editor-in-Chief |
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2020-09-15 23:30 |
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Articles in Press |
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2020-09-15 23:30 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2020-09-20 14:10 |
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Typeset the Manuscript |
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2020-11-19 07:45 |
| ISSN |
1948-5182 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
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