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9/15/2020 11:30:33 PM | Browse: 21 | Download: 31
Publication Name World Journal of Gastroenterology
Manuscript ID 58556
Country/Territory Pakistan
Category Biochemistry and Molecular Biology
Manuscript Type Basic Study
Article Title Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry
Manuscript Source Unsolicited Manuscript
All Author List Mahim Khan, Waqar Rauf, Fazal-e- Habib, Moazur Rahman and Mazhar Iqbal
Funding Agency and Grant Number
Corresponding author Mazhar Iqbal, PhD, Professor, Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Jhang Road,, Faisalabad 38000, Punjab, Pakistan. hamzamgondal@gmail.com
Key Words Hepatitis C virus genotype 3a; Non-structural protein 3 protease; Fluorescence resonance energy transfer assay; Citrus extract; Mass spectrometry; Hesperidin
Core Tip The manuscript describes the screening of active metabolites in citrus fruit extracts against hepatitis c virus genotype3a non-structural protein 3 (HCV-G3a NS3) protease. In this study, conditions have been optimized to get highly purified and functionally active protein HCV NS3. Further, fluorescence resonance energy transfer assay was used to screen the citrus extracts against NS3 protease. By using liquid chromatography coupled with tandem mass spectrometry/ mass spectrometry analysis and bioinformatics modelling approaches, the observed activity of citrus extracts against HCV genotype3a NS3 protease was ascribed to hesperidin. Fluorescence resonance energy transfer assay confirmed the inhibitory potential of hesperidin against NS4A-NS3 protease domain with an IC50 value of 23.32 µM.
Citation Khan M, Rauf W, Habib F, Rahman M, Iqbal M. Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry. World J Gastroenterol 2020; In press
Received
2020-07-27 13:44
Peer-Review Started
2020-07-27 13:44
To Make the First Decision
Return for Revision
2020-08-22 18:43
Revised
2020-09-03 11:38
Second Decision
2020-09-15 12:36
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2020-09-15 23:30
Articles in Press
2020-09-15 23:30
Publication Fee Transferred
Edit the Manuscript by Language Editor
2020-09-20 14:10
Typeset the Manuscript
ISSN 1007-9327 (print) and 2219-2840 (online)
Open Access This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright © The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Assistant Editor Assistant Editor
Science Editor Le Zhang
Reviewer Code 03600138
Editorial Director
Journal Chief Editor
Company Chief Editor Lian-Sheng Ma
Language Editor Donna Fox
Production Editor
Responsible Production Editor
Responsible Science Editor
Proofing Editorial Director Jin-Lei Wang
Proofing Editor-in-Chief Lian-Sheng Ma
Manuscript File 58556-Review-Filipodia.docx
Answering Reviewers 58556-Answering reviewers.PDF
Audio Core Tip 58556-Audio core tip.mp3
Biostatistics Review Certificate 58556-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 58556-Conflict-of-interest statement.pdf
Copyright License Agreement 58556-Copyright license agreement.pdf
Institutional Animal Care and Use Committee Approval Form or Document 58556-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document 58556-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 58556-Language certificate.pdf
Supplementary Material 58556-Supplementary material.pdf
Peer-review Report 58556-Peer-review(s).pdf
Scientific Misconduct Check 58556-Bing-Yan JP-1.png
Scientific Misconduct Check 58556-Scientific misconduct check.pdf
Scientific Editor Work List 58556-Scientific editor work list.pdf