Publication Name	World Journal of Orthopedics
Manuscript ID	72399
Country	Brazil

Category	Orthopedics
Manuscript Type	Prospective Study
Article Title	Quantitative alpha-defensin testing: Is synovial fluid dilution important?
Manuscript Source	Unsolicited Manuscript
All Author List	Rodrigo Calil Teles Abdo, Riccardo Gomes Gobbi, Chilan Bou Ghosson Leite, Sandra Gofinet Pasoto, Elaine Pires Leon, Ana Lucia Lei Munhoz Lima, Eloisa Bonfa, José Ricardo Pécora and Marco Kawamura Demange
Funding Agency and Grant Number	Funding Agency Grant Number Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/25540-9
Corresponding Author	Rodrigo Calil Teles Abdo, MD, Surgeon, Instituto de Ortopedia e Traumatologia, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, R. Dr. Ovídio Pires de Campos, 333 - Cerqueira César., Sao Paulo 05403-010, SP, Brazil. rctabdo@gmail.com
Key Words	Alpha-defensin; Enzyme-linked immunosorbent assay; Periprosthetic joint infection; Laboratory findings
Core Tip	Alpha-defensin is an antimicrobial peptide widely studied in patients with periprosthetic joint infection. Indeed, the analysis of alpha-defensin concentration in the synovial fluid by enzyme-linked immunosorbent assay (ELISA) has been gaining ground. However, there is a lack of information regarding the detailed technique for synovial fluid ELISA, particularly in regard to its dilution. Therefore, this study analyzed the influence of dilution in synovial fluid samples for the alpha-defensin ELISA method. We presume that this novel information may be helpful to make ELISA more reproducible and widely accessible for different treatment centers worldwide.
Citation	Abdo RCT, Gobbi RG, Leite CBG, Pasoto SG, Leon EP, Lima ALLM, Bonfa E, Pécora JR, Demange MK. Quantitative alpha-defensin testing: is synovial fluid dilution important? World J Orthop 2022; 13(8): 760-767

Received	2021-10-15 13:57
Peer-Review Started	2021-10-15 13:58
To Make the First Decision
Return for Revision	2022-01-11 02:45
Revised	2022-02-04 23:13
Second Decision	2022-08-01 03:08
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2022-08-05 22:01
Articles in Press	2022-08-05 22:01
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2022-08-12 05:45

ISSN	2218-5836 (online)
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