Publication Name	World Journal of Gastroenterology
Manuscript ID	82959
Country	Romania

Category	Gastroenterology & Hepatology
Manuscript Type	Observational Study
Article Title	Endoscopic ultrasound-guided fine-needle aspiration pancreatic adenocarcinoma samples yield adequate DNA for next-generation sequencing: A cohort analysis
Manuscript Source	Unsolicited Manuscript
All Author List	Stefania Bunduc, Bianca Varzaru, Razvan Andrei Iacob, Andrei Sorop, Ioana Manea, Andreea Spiridon, Raluca Chelaru, Adina Emilia Croitoru, Gabriel Becheanu, Mona Dumbrava, Simona Dima, Irinel Popescu and Cristian Gheorghe
Funding Agency and Grant Number
Corresponding Author	Cristian Gheorghe, FASGE, MD, PhD, Professor, Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, No. 258 Fundeni Road, Bucharest 020021, Romania. drcgheorghe@gmail.com
Key Words	Pancreatic adenocarcinoma; Endoscopic ultrasound guided fine needle aspiration; Next generation sequencing; DNA yield; Needle size; Genetic testing
Core Tip	Genetic testing is increasingly undertaken in pancreatic adenocarcinoma (PDAC). The main diagnostic method is endoscopic ultrasound (EUS)-guided tissue acquisition and fine needle aspiration (FNA) is most frequently performed in clinical practice. However, current guidelines recommend using core specimens for genotyping aimed samples. In our cohort analysis, we show that EUS-FNA PDAC samples yield DNA of adequate amount and purity for subsequent next-generation sequencing (NGS). DNA amount was similar between 22G and 19G FNA needles. DNA purity parameters may vary indirectly with needle size. EUS FNA PDAC samples may be used for NGS.
Citation	Bunduc S, Varzaru B, Iacob RA, Sorop A, Manea I, Spiridon A, Chelaru R, Croitoru AE, Becheanu G, Dumbrava M, Dima S, Popescu I, Gheorghe C. Endoscopic ultrasound-guided fine-needle aspiration pancreatic adenocarcinoma samples yield adequate DNA for next-generation sequencing: A cohort analysis. World J Gastroenterol 2023; 29(18): 2864-2874

Received	2022-12-31 21:08
Peer-Review Started	2022-12-31 21:11
To Make the First Decision
Return for Revision	2023-02-28 05:26
Revised	2023-03-14 00:26
Second Decision	2023-04-18 02:58
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2023-04-18 07:14
Articles in Press	2023-04-18 07:14
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2023-04-26 06:33

ISSN	1007-9327 (print) and 2219-2840 (online)
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