Publication Name	World Journal of Stem Cells
Manuscript ID	101290
Country	China

Category	Cell & Tissue Engineering
Manuscript Type	Basic Study
Article Title	RNA interference-mediated osteoprotegerin silencing increases the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin ratio and promotes osteoclastogenesis
Manuscript Source	Unsolicited Manuscript
All Author List	Song-Guan Wei, Hui-Hong Chen, Liu-Rong Xie, Yuan Qin, Yu-Ying Mai, Lin-Hui Huang and Hong-Bing Liao
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 82160192 Guangxi Science and Technology Program 2023AB23037
Corresponding Author	Hong-Bing Liao, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, College & Hospital of Stomatology, Guangxi Medical University, No. 22 Shuangyong Road, Qingxiu District, Nanning 530021, Guangxi Zhuang Autonomous Region, China. hongbing_liao@gxmu.edu.cn
Key Words	Osteoprotegerin; Receptor activator of nuclear factor-kappa B ligand; Bone marrow-derived mesenchymal stem cells; RNA interference; Osteoclast; Bone scaffold
Core Tip	This study reports, for the first time, that enhancing the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin (RANKL/OPG) ratio through RNA interference promotes osteoclastogenesis. Our findings reveal a significant upregulation of RANKL mRNA levels after OPG gene silencing. The study demonstrates a significant downregulation of OPG mRNA and protein levels. The increase in the RANKL/OPG ratio significantly promotes osteoclastogenesis. This study provides a new theoretical basis and molecular targets for degrading bone scaffolds and bone tissue repair.
Citation	<p>Wei SG, Chen HH, Xie LR, Qin Y, Mai YY, Huang LH, Liao HB. RNA interference-mediated osteoprotegerin silencing increases the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin ratio and promotes osteoclastogenesis. <i>World J Stem Cells</i> 2025; 17(4): 101290</p>

Received	2024-09-10 03:29
Peer-Review Started	2024-09-10 03:29
To Make the First Decision
Return for Revision	2024-10-28 08:16
Revised	2024-12-13 09:22
Second Decision	2025-02-18 02:43
Accepted by Journal Editor-in-Chief	2025-02-19 01:15
Accepted by Executive Editor-in-Chief	2025-03-21 11:01
Articles in Press	2025-03-21 11:01
Publication Fee Transferred	2024-12-23 03:47
Edit the Manuscript by Language Editor
Typeset the Manuscript	2025-04-10 03:33

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright	© The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com