Publication Name	World Journal of Stem Cells
Manuscript ID	104116
Country	China

Category	Cell & Tissue Engineering
Manuscript Type	Basic Study
Article Title	Optimal good manufacturing practice-compliant production of dental follicle stem cell sheet and its application in Sprague-Dawley rat periodontitis
Manuscript Source	Unsolicited Manuscript
All Author List	Jia-Lu Yu, Chao Yang, Li Liu, An Lin, Shu-Juan Guo and Wei-Dong Tian
Funding Agency and Grant Number	Funding Agency Grant Number National Key Research and Development Program of China 2021YFA1100600, 2022YFA1104400
Corresponding Author	Wei-Dong Tian, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Engineering Research Center of Oral Translational Medicine, National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Renmin South Road, Wuhou District, Chengdu 610041, Sichuan Province, China. drtwd@sina.com
Key Words	Periodontitis; Dental follicle stem cells; Cell sheet; Periodontal tissue regeneration; Mesenchymal stem cells; Vitamin C; Cell concentration; L-ascorbic acid
Core Tip	The existing culture method for dental follicle stem cell (DFSC) sheets is crude and not uniform. For clinical application, it is necessary to produce good manufacturing practice compliant DFSC sheets and determine a uniform culture method that can maintain the best performance of DFSC sheets and consume the least. In this study, the culture conditions of the good manufacturing practice-compliant DFSC sheet were optimized, focusing on four key factors: Cell passage, cell concentration, L-ascorbic acid content, and culture duration. Compared with the DFSC suspension, the cell sheet demonstrated superior capacities. These findings offer valuable insights and guidance for the clinical application of cell sheets.
Citation	Yu JL, Yang C, Liu L, Lin A, Guo SJ, Tian WD. Optimal good manufacturing practice-compliant production of dental follicle stem cell sheet and its application in Sprague-Dawley rat periodontitis. World J Stem Cells 2025; In press

Received	2024-12-11 04:10
Peer-Review Started	2024-12-16 00:47
To Make the First Decision
Return for Revision	2025-02-14 08:37
Revised	2025-02-27 20:36
Second Decision	2025-04-08 00:42
Accepted by Journal Editor-in-Chief	2025-04-08 07:26
Accepted by Executive Editor-in-Chief	2025-04-18 08:33
Articles in Press	2025-04-18 08:33
Publication Fee Transferred	2025-03-03 05:39
Edit the Manuscript by Language Editor
Typeset the Manuscript	2025-05-05 11:10

ISSN	1948-0210 (online)
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