Publication Name	World Journal of Diabetes
Manuscript ID	105228
Country	China

Category	Urology & Nephrology
Manuscript Type	Basic Study
Article Title	Activation of farnesoid X receptor upregulates binding immunoglobulin protein expression and alleviates diabetic nephropathy
Manuscript Source	Unsolicited Manuscript
All Author List	Jian-Ying Tang, Yuan-Jia Chong, Lu Yang, Xue Li, Ying Yang, Jun-Chen Li and Jiao Mu
Funding Agency and Grant Number	Funding Agency Grant Number Talent Launch Fund of Chongqing Medical University Affiliated University City Hospital Chongqing Medical University Affiliated University City Hospital Youth Program No. 2021ZD05
Corresponding Author	Jun-Chen Li, Department of Nephrology, University-Town Hospital of Chongqing Medical University, No. 55 Daxuecheng Middle Road, Shapingba District, Chongqing 401331, China. 8a3610@hospital.cqmu.edu.cn
Key Words	Binding immunoglobulin protein; Chromatin immunoprecipitation; Diabetic nephropathy; Endoplasmic reticulum stress; Farnesoid X receptor
Core Tip	What was known: Farnesoid X receptor (FXR) agonists alleviate proteinuria and glomerulosclerosis in diabetic nephropathy mice by inhibiting inflammation and oxidative stress. Hence, their protective effects have become a research focus in recent years. However, the specific targets and signaling pathways involved in their regulation remain unclear. This study adds: We demonstrated for the first time that using bioinformatics prediction, protein immunoprecipitation, and luciferase reporter assays that FXR directly binds to the promoter region of the binding immunoglobulin protein (BiP) gene. FXR activates the transcriptional activity of BiP, thereby explaining the molecular mechanism of FXR-regulated BiP transcription. We confirmed through in vitro experiments that FXR agonist obeticholic acid (INT-747) administration significantly promotes BiP expression in high-glucose-induced SV40 MES13 cells, while FXR inhibitors downregulated BiP expression. Potential impact: FXR has a direct regulatory effect on BiP. This study provides valuable insights regarding the prevention and treatment of diabetic nephropathy.
Citation	Tang JY, Chong YJ, Yang L, Li X, Yang Y, Li JC, Mu J. Activation of farnesoid X receptor upregulates binding immunoglobulin protein expression and alleviates diabetic nephropathy. World J Diabetes 2025; In press

Received	2025-01-15 11:05
Peer-Review Started	2025-01-16 01:00
To Make the First Decision
Return for Revision	2025-03-10 02:28
Revised	2025-03-23 16:40
Second Decision	2025-06-11 06:13
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-06-11 08:35
Articles in Press	2025-06-11 08:35
Publication Fee Transferred	2025-03-28 07:38
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Typeset the Manuscript

ISSN	1948-9358 (online)
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