| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Chaperonin-containing tailless complex polypeptide 1 subunit 6A negatively regulates autophagy and protects colorectal cancer cells from cisplatin-induced cytotoxicity
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Jian-Xing Ma, Xiao-Jun Li, Ya-Long Li, Ming-Chan Liu, Rui-Hang Du, Yi Cheng, Liang-Jie Li, Zhi-Ying Ai, Jian-Tao Jiang and Si-Yuan Yan |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Shandong Provincial Natural Science Foundation |
ZR2023MH329 |
| Project of Shandong Province Higher Educational Youth Innovation Science and Technology Program |
2023KJ263 |
| Natural Science Foundation of Gansu Province, China |
22JR5RA953 |
|
| Corresponding Author |
Si-Yuan Yan, Associate Professor, PhD, Precision Medicine Laboratory for Chronic Non-communicable Diseases of Shandong Province, Institute of Precision Medicine, Jining Medical University, No. 133 Hehua Road, Jining 272067, Shandong Province, China. yansy@mail.jnmc.edu.cn |
| Key Words |
Chaperonin-containing tailless complex polypeptide 1 subunit 6a; Cisplatin; Autophagy; Colorectal cancer; Necroptosis |
| Core Tip |
This study delves into the role of chaperonin-containing tailless complex polypeptide 1 subunit 6A (CCT6A), a protein folding chaperone, in CRC therapy. Our findings reveal that CCT6A expression is downregulated by cisplatin (DDP) in CRC cells, enhancing DDP's cytotoxicity. Depletion of CCT6A further potentiates DDP's effects by promoting autophagy, apoptosis, and necroptosis, while its overexpression provides resistance. Using immunoblotting and autophagy assays, we confirmed CCT6A's negative regulatory role in autophagy. Markers of apoptosis and necroptosis were upregulated in CCT6A-depleted cells, indicating increased cell death. Autophagy inhibition exacerbated cell death, compromising viability and proliferation. Thus, CCT6A emerges as a potential therapeutic target in CRC, modulating key cellular processes and enhancing DDP efficacy. |
| Citation |
Ma JX, Li XJ, Li YL, Liu MC, Du RH, Cheng Y, Li LJ, Ai ZY, Jiang JT, Yan SY. Chaperonin-containing tailless complex polypeptide 1 subunit 6A negatively regulates autophagy and protects colorectal cancer cells from cisplatin-induced cytotoxicity. World J Gastroenterol 2025; In press |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
|
| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
