Publication Name	World Journal of Gastrointestinal Oncology
Manuscript ID	106663
Country	Singapore

Category	Oncology
Manuscript Type	Minireviews
Article Title	Challenges and proposed solutions to the adoption of cell free DNA in screening, detecting and prognosticating colorectal cancer
Manuscript Source	Unsolicited Manuscript
All Author List	Megan Wern-Ee Chua and Dedrick Kok-Hong Chan
Funding Agency and Grant Number
Corresponding Author	Dedrick Kok-Hong Chan, Department of Colorectal Surgery, National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074, Singapore. surckhd@nus.edu.sg
Key Words	Cell free DNA; Circulating tumour deoxyribonucleic acid; Colorectal cancer; Liquid biopsy; Screening; Detection; Prognostication
Core Tip	Cell free DNA (cfDNA) carries information about colorectal cancer-specific genetic and epigenetic alterations which can aid in screening, detection and prognostication. Detection of genetic alterations is made difficult by low signal-to-noise ratio owing to an abundance of background non-tumorigenic mutations. Low amounts of cfDNA in healthy individuals negatively affects the sequencing performance and limit of detection of assays making screening non-feasible. One solution is to harvest cfDNA from peritoneal fluid or stool as this is more representative of the primary tumour compared to plasma-derived cfDNA. Alternatively, increasing the sensitivity of sequencing technologies would allow for the detection of low frequency mutations.
Citation	Chua MWE, Chan DKH. Challenges and proposed solutions to the adoption of cell free DNA in screening, detecting and prognosticating colorectal cancer. World J Gastrointest Oncol 2025; In press

Received	2025-03-04 05:17
Peer-Review Started	2025-03-04 05:17
To Make the First Decision
Return for Revision	2025-04-08 09:41
Revised	2025-04-08 16:14
Second Decision	2025-07-04 02:35
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-07-04 08:50
Articles in Press	2025-07-04 08:50
Publication Fee Transferred	2025-04-13 07:34
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-5204 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright	© The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
Permissions	For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com