Publication Name	World Journal of Hepatology
Manuscript ID	107329
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Chromatin accessibility module identified by single-cell sequencing underlies the diagnosis and prognosis of hepatocellular carcinoma
Manuscript Source	Unsolicited Manuscript
All Author List	Xiao-Li Xi, Yi-Dong Yang, Hui-Ling Liu, Jie Jiang and Bin Wu
Funding Agency and Grant Number	Funding Agency Grant Number Science and Technology Planning Project of Guangzhou No. 2024A03J0102 Natural Science Foundation of Guangdong Province for Distinguished Young Scholar No. 2022B1515020024 National Natural Science Foundation of China No. 82070574 Key Research and Development Program of Guangzhou No. 2023B03J1298
Corresponding Author	Bin Wu, Department of Gastroenterology, The Third Affiliated Hospital of Sun Yat-Sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China. wubin6@mail.sysu.edu.cn
Key Words	Hepatocellular carcinoma; Single-cell multiomics; Chromatin accessibility; Enhancer-like peak; Chromatin coaccessibility
Core Tip	This study unveils multilayered chromatin accessibility dynamics in hepatocellular carcinoma. Single-cell multiomics analysis discovered malignant hepatocytes adopting increased numbers of accessible peaks and larger physical regions despite reduced peak intensity, while tumor-stroma intercellular chromatin coaccessibility maintains tumor ecological symbiosis. Functional validation identified DGAT1 as an epigenetic vulnerability: Pharmacological histone 3 lysine 27 acetylation inhibition and relating decreased chromatin accessibility suppressed DGAT1 expression, and DGAT1 knockdown further blocked tumor growth. Our findings redefine hepatocellular carcinoma progression as a chromatin-adaptive disorder governed by tumor-intrinsic epigenetic prioritization and microenvironmental crosstalk, proposing simultaneous targeting of chromatin hub genes and compensatory pathways as precision strategies.
Citation	Xi XL, Yang YD, Liu HL, Jiang J, Wu B. Chromatin accessibility module identified by single-cell sequencing underlies the diagnosis and prognosis of hepatocellular carcinoma. World J Hepatol 2025; In press

Received	2025-03-21 02:05
Peer-Review Started	2025-03-31 00:10
To Make the First Decision
Return for Revision	2025-04-11 09:57
Revised	2025-04-24 17:30
Second Decision	2025-05-29 02:54
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-05-29 10:19
Articles in Press	2025-05-29 10:19
Publication Fee Transferred	2025-04-26 13:31
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-5182 (online)
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