Publication Name	World Journal of Gastrointestinal Surgery
Manuscript ID	109521
Country	China

Category	Surgery
Manuscript Type	Case Control Study
Article Title	Gallstone and bile microbiota: A case-control study based on 16S rRNA gene sequencing
Manuscript Source	Unsolicited Manuscript
All Author List	Zhong-Xiao Lu, Yan-Qi Jiang, Dang-Shou Wang, Yu-Ting Song, Xiao-Ming Jiang, Fu-Jian Xu, Jie Tang, Bing Li and Wen-Hai Huang
Funding Agency and Grant Number	Funding Agency Grant Number Youth Scientific Research Launch Fund Project of Jinshan Hospital, Affiliated with Fudan University No. JYQN-LC-202214
Corresponding Author	Bing Li, Researcher, Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, No. 1508 Longhang Road, Shanghai 201508, China. li_bing@fudan.edu.cn
Key Words	Gallstones; Bile microbiota; 16S rRNA sequencing; Diversity analysis; Genus identification
Core Tip	This retrospective study analyzed bile microbiota composition in 40 patients. Results demonstrated that samples from gallstone patients exhibited significantly reduced overall microbial diversity alongside a striking dominance of Proteobacteria. In contrast, bile samples from gallstone-free individuals showed relative enrichment in health-associated genera, including protective taxa like Lactobacillus. The findings indicate that dysbiosis within the bile microbiome, particularly the overgrowth of Proteobacteria, actively drives gallstone pathogenesis. This suggests that modulating the bile microbiota represents a promising novel therapeutic avenue for gallstone prevention or treatment through targeted interventions.
Citation	Lu ZX, Jiang YQ, Wang DS, Song YT, Jiang XM, Xu FJ, Tang J, Li B, Huang WH. Gallstone and bile microbiota: A case-control study based on 16S rRNA gene sequencing. World J Gastrointest Surg 2025; In press

Received	2025-05-14 02:52
Peer-Review Started	2025-05-14 02:52
To Make the First Decision
Return for Revision	2025-05-22 09:55
Revised	2025-06-04 18:36
Second Decision	2025-07-18 02:43
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2025-07-18 05:35
Articles in Press	2025-07-18 05:35
Publication Fee Transferred	2025-06-09 11:32
Edit the Manuscript by Language Editor
Typeset the Manuscript

ISSN	1948-9366 (online)
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