Publication Name	World Journal of Stem Cells
Manuscript ID	89404
Country	Brazil

Category	Biochemistry & Molecular Biology
Manuscript Type	Basic Study
Article Title	RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation
Manuscript Source	Invited Manuscript
All Author List	Daniel B Ferreira, Leticia M Gasparoni, Cristiane F Bronzeri and Katiucia BS Paiva
Funding Agency and Grant Number	Funding Agency Grant Number São Paulo Research Foundation (FAPESP) 2010/08918-9, 2020/11564-6 KBSP Young Investigator Fellowship 2011/00204-0 DBF Fellowship 2019/27492-7 LMG Fellowship 2014/01395-1 CFB Fellowship 2014/14278-3
Corresponding Author	Katiucia BS Paiva, BSc, PhD, Assistant Professor, Laboratory of Extracellular Matrix Biology and Cellular Interaction, Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes n. 2.415 (ICB3 - laboratório 111), São Paulo 05508-000, São Paulo, Brazil. katipaiva@usp.br
Key Words	Dental pulp stem cells; Reference gene; Housekeeping gene; Endogenous gene; Osteogenic differentiation; RefFinder
Core Tip	Detecting the best reference genes (RG) under specific conditions has been recommended as a good practice to improve the understanding of gene expression. Stem cells have been largely studied during commitment to particular cell lineages for many applications, such as tissue engineering. In this way, dental pulp stem cells (DPSCs) are a promise for craniofacial reconstruction. For the first time, we show that the best pair of RGs for osteogenic differentiation of human DPSCs are (ribosomal protein, large, P0)/(TATA box binding protein) by quantitative real-time polymerase chain reaction through the four algorithms (ΔCt comparative method, GeNorm, BestKeeper, and NormFinder) and ranked by RefFinder.
Citation	<p>Ferreira DB, Gasparoni LM, Bronzeri CF, Paiva KB. RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation. <i>World J Stem Cells</i> 2024; 16(6): 656-669</p>

Received	2023-11-23 12:57
Peer-Review Started	2023-11-23 12:57
To Make the First Decision
Return for Revision	2023-12-21 21:52
Revised	2024-01-23 18:28
Second Decision	2024-04-01 01:40
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2024-04-12 06:53
Articles in Press	2024-04-12 06:53
Publication Fee Transferred
Edit the Manuscript by Language Editor	2024-04-19 03:32
Typeset the Manuscript	2024-05-23 11:55

ISSN	1948-0210 (online)
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