Publication Name	World Journal of Gastrointestinal Oncology
Manuscript ID	91633
Country	China

Category	Gastroenterology & Hepatology
Manuscript Type	Basic Study
Article Title	Shi-pi-xiao-ji formula suppresses hepatocellular carcinoma by reducing cellular stiffness through upregulation of acetyl-coA acetyltransferase 1
Manuscript Source	Unsolicited Manuscript
All Author List	Hui-Ying Jian, Zi-Cheng Liang, Huan Wen, Zhen Zhang and Pu-Hua Zeng
Funding Agency and Grant Number	Funding Agency Grant Number National Natural Science Foundation of China 82074425 Hunan Science and Technology Planning Project 2016SK2051, 2023SK2057 Hunan Provincial Administration of Traditional Chinese Medicine Research Project B2023089
Corresponding Author	Zhen Zhang, PhD, Doctor, Hunan Provincial Hospital of Integrated Traditional Chinese and Western, Cancer Research Institute of Hunan Academy of Traditional Chinese Medicine, Hunan Academy of Chinese Medicine, No. 58 Lushan Road, Changsha 410208, Hunan Province, China. zhangzhen@stu.hnucm.edu.cn
Key Words	Shi-pi-xiao-ji formula; Hepatocellular carcinoma; Cellular stiffness; Plasma membrane cholesterol level; Acetyl-coA acetyltransferase 1
Core Tip	Cellular stiffness is a key feature of cellular biomechanics and is determined by cholesterol levels in the plasma membrane. Traditional Chinese medicine (TCM) has demonstrated good anti-tumor effects, but underlying mechanisms are not well characterized. Herein, we investigated the effects of the Shi-pi-xiao-ji (SPXJ) herbal formula on the stiffness of hepatocellular carcinoma (HCC) cells. The SPXJ formula altered the stiffness of HCC cells by decreasing plasma membrane cholesterol levels through upregulation of acetyl-coA acetyltransferase 1. Subsequently, this inhibited the invasion and metastasis of HCC cells. Our study provides a scientific basis for the clinical use of TCM preparations in the treatment of HCC.
Citation	Jian HY, Liang ZC, Wen H, Zhang Z, Zeng PH. Shi-pi-xiao-ji formula suppresses hepatocellular carcinoma by reducing cellular stiffness through upregulation of acetyl-coA acetyltransferase 1. World J Gastrointest Oncol 2024; In press

Received	2024-01-01 09:08
Peer-Review Started	2024-01-01 09:08
To Make the First Decision
Return for Revision	2024-02-25 11:20
Revised	2024-03-14 13:17
Second Decision	2024-04-23 02:45
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2024-04-23 07:45
Articles in Press	2024-04-23 07:45
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ISSN	1948-5204 (online)
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