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6/16/2026 8:20:03 AM | Browse: 2 | Download: 0
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2025-12-28 14:34 |
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2026-03-02 02:40 |
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2026-03-02 03:47 |
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Articles in Press |
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2026-03-02 03:47 |
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Typeset the Manuscript |
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2026-06-04 07:01 |
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Publish the Manuscript Online |
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2026-06-16 08:10 |
| ISSN |
2220-315x (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Quantitative assessment of STAT3 and HPV16 E6 transcripts using Flow-FISH approach for early detection of progressive cervical lesions
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Arun Chhokar, Udit Joshi, Tanya Tripathi, Bindiya Gupta, Madeeha Mudassir, Divya Janjua, Apoorva Chaudhary, Joni Yadav, Nikita Aggarwal, Vinita K Jaggi and Alok C Bharti |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Indian Council of Medical Research |
No. 5/13/4/ACB/ICRC/2020/NCD-III |
| Indian Council of Medical Research |
No. SG/Dev. Res/05750/2025-2028 (261835) |
| Indian Council of Medical Research AdHOC |
No. 2021-10573/GENOMIC/ADHOC-BMS |
| Indian Council of Medical Research AdHOC |
No. IG/Dev.Res/00265/2029-2025 (256963) |
| Central Council for Research in Homoeopathy, Ministry of AYUSH, Government of India, No. 17-30/2023-24/CCRH/Tech./Coll./DO-Cervical Cancer Phase-II/898 |
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| Institution of Eminence, University of Delhi |
No. /IoE/2025-26/12/FRP |
| ANRF |
No. ANRF/PAIR/2025/000003/PAIR-B |
| University Grants Commission |
No. 764/(CSIR-UGC NET JUNE 2019) |
| Council of Scientific and Industrial Research |
No. 09/0045/(11635)/2021-EMR-1 |
| ANRF |
No. 73(CSIR-UGC NET JUNE 2017) |
| Council of Scientific and Industrial Research |
No. 09/0045(12901)/2022-EMR-1 |
| Council of Scientific and Industrial Research |
No. 09/045(1629)/2019-EMR-I |
| Council of Scientific and Industrial Research |
No. 09/045(1622)/2018-EMR-I |
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| Corresponding Author |
Alok C Bharti, PhD, Professor, Department of Zoology, University of Delhi, Gate No. 3 Chaatra Marg, University of Delhi North Campus, Delhi 110007, India. alokchandrab@yahoo.com |
| Key Words |
Cervical cancer; Human papillomavirus; Signal transducer and activator of transcription 3; Fluorescence in-situ hybridization; Flow-cytometry |
| Core Tip |
This study evaluates the feasibility of translating a microscopy-optimized fluorescence in situ hybridization (FISH) assay for signal transducer and activator of transcription 3 and human papillomavirus (HPV) 16 E6 transcripts into a flow cytometry-based FISH (Flow-FISH). While microscopy-based FISH reliably detected transcripts in cervical cancer cell lines, pre-published probes demonstrated limited sensitivity and specificity in Flow-FISH, particularly for HPV16 E6. These findings highlight critical technical bottlenecks in adapting slide-based FISH assays to flow-cytometry and underscore the need for platform-specific probe design and assay re-optimization before Flow-FISH can be applied as a scalable and objective screening tool for HPV-associated cervical cancer. |
| Publish Date |
2026-06-16 08:10 |
| Citation |
Chhokar A, Joshi U, Tripathi T, Gupta B, Mudassir M, Janjua D, Chaudhary A, Yadav J, Aggarwal N, Jaggi VK, Bharti AC. Quantitative assessment of STAT3 and HPV16 E6 transcripts using Flow-FISH approach for early detection of progressive cervical lesions. World J Exp Med 2026; 16(2): 118250
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| URL |
https://www.wjgnet.com/2220-315X/full/v16/i2/118250.htm |
| DOI |
https://doi.org/10.5493/wjem.v16.i2.118250 |
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