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6/16/2026 8:20:03 AM | Browse: 2 | Download: 0
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Received |
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2026-01-26 01:45 |
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Peer-Review Started |
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2026-01-26 01:46 |
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First Decision by Editorial Office Director |
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2026-02-10 10:28 |
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2026-02-10 10:28 |
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Revised |
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2026-02-13 05:24 |
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Publication Fee Transferred |
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Second Decision by Editor |
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2026-04-02 02:34 |
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Second Decision by Editor-in-Chief |
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Final Decision by Editorial Office Director |
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2026-04-02 08:12 |
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Articles in Press |
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2026-04-02 08:12 |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2026-05-28 08:12 |
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Publish the Manuscript Online |
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2026-06-16 08:10 |
| ISSN |
2220-315x (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
©Author(s) (or their employer(s)) 2026. No commercial re-use. See Permissions. Published by Baishideng Publishing Group Inc. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Medicine, General & Internal |
| Manuscript Type |
Prospective Study |
| Article Title |
Comparative evaluation of staining protocols for detecting intracellular bacterial communities and biofilm formation in UTIs with E. coli
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Swathi Pandey, Arul Senghor Kadalangudi Aravaanan, Emmanuel Bhaskar and Santhi Silambanan |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Founder Chancellor Shri. N.P.V. Ramasamy Udayar Research Fellowship, SRIHER |
No. U022300967 |
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| Corresponding Author |
Santhi Silambanan, MD, Professor, Department of Biochemistry, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai 600116, Tamil Nadu, India. santhisilambanan@sriramachandra.edu.in |
| Key Words |
Urinary tract infections; Intracellular bacterial communities; Biofilm formation; Sustainable Development Goals-3; Escherichia coli |
| Core Tip |
Escherichia coli urinary tract infections (UTIs) are persistent through formation of intracellular bacterial communities (IBCs) and biofilms, which are not detected by conventional diagnostic techniques. This work was designed to compare Sternheimer-Malbin, Wright-Giemsa, Safranin, and immunofluorescent staining methods for the detection of IBCs in urinary sediments. Compared with Wright-Giemsa and Sternheimer-Malbin, immunofluorescence was proven to have a higher detection rate for diagnosing IBCs. The results underscore the importance of novel diagnostic procedures and therapeutic strategies directed not only towards intracellular but also biofilm-embedded bacteria for the successful treatment of UTIs. |
| Publish Date |
2026-06-16 08:10 |
| Citation |
Pandey S, Aravaanan ASK, Bhaskar E, Silambanan S. Comparative evaluation of staining protocols for detecting intracellular bacterial communities and biofilm formation in UTIs with E. coli. World J Exp Med 2026; 16(2): 119306
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| URL |
https://www.wjgnet.com/2220-315x/full/v16/i2/119306.htm |
| DOI |
https://doi.org/10.5493/wjem.v16.i2.119306 |
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