| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell & Tissue Engineering |
| Manuscript Type |
Basic Study |
| Article Title |
Differentiation of patient-specific induced pluripotent stem cells derived from type 1 diabetes peripheral blood mononuclear cells into pancreatic β-like cells
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Kun Wang, Wei Lin, Jun-Yong Han, Jin-Yan Chen, Rong-Hua Liu, Zhen Yu and Jing-Jun Jin |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Nonprofit Research Institutes Foundation of Fujian Province, China |
2020R1011003, 2022R1012001 |
| Talents Training Project for the Key Young Scholars of Fujian Provincial Health Commission, China |
2021GGA056 |
|
| Corresponding Author |
Kun Wang, Associate Professor, PhD, Fujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, No. 7 Wusi Road, Fuzhou 350001, Fujian Province, China. wangkun973@fjms.ac.cn |
| Key Words |
Pluripotent stem cells; Cell reprogramming; Type 1 diabetes; Triiodothyronine; Vitamin C; Adenovirus; β-cell regeneration; Differentiation |
| Core Tip |
This study successfully employed an episomal plasmid system to reprogram patient peripheral blood mononuclear cells into type 1 diabetes (T1D)-induced pluripotent stem cells (iPSCs). Optimization of the differentiation protocol, utilizing triiodothyronine (T3), vitamin C (Vc), and adenovirus-M3C, facilitated the directed differentiation of T1D-iPSCs towards pancreatic β-cells. The T3 + Vc group exhibited minimal neurogenin 3 expression, while the M3C + T3 + Vc group demonstrated maximal MAF bZIP transcription factor A expression. While insulin⁺ β-like cells differentiated from T1D-iPSCs displayed basal insulin secretion, they lacked the ability to respond to glucose stimulation, indicating functional immaturity compared to fully mature β-cells. These results underscore their potential as seed cells for β-cell replacement therapy in T1D. |
| Publish Date |
2025-07-24 09:11 |
| Citation |
<p>Wang K, Lin W, Han JY, Chen JY, Liu RH, Yu Z, Jin JJ. Differentiation of patient-specific induced pluripotent stem cells derived from type 1 diabetes peripheral blood mononuclear cells into pancreatic β-like cells. <i>World J Stem Cells</i> 2025; 17(7): 104607</p> |
| URL |
https://www.wjgnet.com/1948-0210/full/v17/i7/104607.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v17.i7.104607 |
