Publication Name	World Journal of Gastroenterology
Manuscript ID	14519
Country	Ireland

Received	2014-10-09 09:59
Peer-Review Started	2014-10-09 14:33
First Decision by Editorial Office Director
Return for Revision	2014-11-20 09:10
Revised	2014-11-28 17:49
Publication Fee Transferred
Second Decision by Editor	2014-12-16 15:26
Second Decision by Editor-in-Chief	2014-12-18 10:19
Final Decision by Editorial Office Director	2014-12-22 17:02
Articles in Press	2014-12-22 17:24
Edit the Manuscript by Language Editor
Typeset the Manuscript	2015-04-24 14:25
Publish the Manuscript Online	2015-05-18 17:02

ISSN	1007-9327 (print) and 2219-2840 (online)
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Category	Surgery
Manuscript Type	Clinical Trials Study
Article Title	Decreased expression of hyperpolarisation-activated cyclic nucleotide-gated channel 3 in Hirschsprung’s disease
Manuscript Source	Unsolicited Manuscript
All Author List	Anne Marie O’Donnell, David Coyle and Prem Puri
Funding Agency and Grant Number	Funding Agency Grant Number National Children’s Research Centre/Children’s Medical Research Foundation, Ireland
Corresponding Author	Prem Puri, Professor, MS, FRCS, FRCS (Ed), FACS, FAAP (Hon.), DSc (Hon.), National Children’s Research Centre, Our Lady’s Children’s Hospital, Cooley Rd, Crumlin, Dublin 12, Ireland. prem.puri@ucd.ie
Key Words	Hyperpolarisation-activated nucleotide-gated; Interstitial cells of Cajal; Hirschsprung’s; Pacemaker; Ih current
Core Tip	Children with Hirschsprung’s disease experience symptoms of intestinal obstruction due to a spastically contracted aganglionic segment of distal bowel, however the mechanism for this spasticity is incompletely under?stood. Hyperpolarisation-activated nucleotide-gated channels play a key role in regulating cell excitability in both pacemaker and non-pacing cells. We examined expression of hyperpolarisation-activated nucleotide-gated (HCN) 1-4 in both Hirschsprung’s disease (HSCR) colon and healthy controls. While HCN-1 expression was absent, and HCN-2 and HCN-4 were expressed at similar levels in diseased and healthy bowel, we found HCN-3 to be reduced in aganglionic bowel in HSCR. We suggest deficient HCN3 channels may be involved in the pathophysiology of HSCR.
Publish Date	2015-05-18 17:02
Citation	O’Donnell AM, Coyle D, Puri P. Decreased expression of hyperpolarisation-activated cyclic nucleotide-gated channel 3 in Hirschsprung’s disease. World J Gastroenterol 2015; 21(18): 5635-5640
URL	http://www.wjgnet.com/1007-9327/full/v21/i18/5635.htm
DOI	http://dx.doi.org/10.3748/wjg.v21.i18.5635

Full Article (PDF)	WJG-21-5635.pdf
Full Article (Word)	WJG-21-5635.doc