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11/23/2015 2:56:00 PM | Browse: 582 | Download: 1872
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Received |
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2015-04-29 21:53 |
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Peer-Review Started |
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2015-04-30 22:15 |
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To Make the First Decision |
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2015-08-16 16:54 |
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Return for Revision |
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2015-08-20 11:02 |
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Revised |
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2015-09-01 00:47 |
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Second Decision |
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2015-09-21 17:06 |
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Accepted by Journal Editor-in-Chief |
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2015-09-21 20:45 |
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Accepted by Company Editor-in-Chief |
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2015-09-30 18:16 |
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Articles in Press |
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2015-09-30 18:16 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2015-11-04 09:45 |
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Publish the Manuscript Online |
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2015-11-23 10:19 |
| ISSN |
1949-8454 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Biochemistry & Molecular Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Disruption of NAD+ binding site in glyceraldehyde 3-phosphate dehydrogenase affects its intranuclear interactions
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Manali Phadke, Natalia Krynetskaia, Anurag Mishra, Carlos Barrero, Salim Merali, Scott A Gothe and Evgeny Krynetskiy |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Cancer Institute |
R01-CA104729 |
| Jayne Haines Center for Pharmacogenomics and Drug Safety of Temple University School of Pharmacy and Temple University Summer Research Award |
to Evgeny Krynetskiy |
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| Corresponding Author |
Evgeny Krynetskiy, PhD, DSc, Associate Professor, Temple University School of Pharmacy, 3307 North Broad Street, Philadelphia, PA 19140,
United States. ekrynets@temple.edu
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| Key Words |
NAD+; Binding site; Fluorescence recovery after photobleaching; Nuclear proteins; Mutation; Glycer¬aldehyde 3-phosphate dehydrogenase; Anticancer agents |
| Core Tip |
We detected the phosphorylated amino acid residues Y94, S98, T99 within the NAD+ binding center of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Substitution of these amino acids with non-phosphorylated alanine residues did not abrogate intranuclear localization of GAPDH. Instead, such mutations altered the molecular dynamics parameters of intranuclear GAPDH probably by hindering its interactions with yet to be identified nuclear biomolecules. Our molecular modeling experiments invoke an important structural feature -T99-E97 H-bond likely involved in stabilization of NAD+ binding center. |
| Publish Date |
2015-11-23 10:19 |
| Citation |
Phadke M, Krynetskaia N, Mishra A, Barrero C, Merali S, Gothe SA, Krynetskiy E. Disruption of NAD+ binding site in glyceraldehyde 3-phosphate dehydrogenase affects its intranuclear interactions. World J Biol Chem 2015; 6(4): 366-378 |
| URL |
http://www.wjgnet.com/1949-8454/full/v6/i4/366.htm |
| DOI |
http://dx.doi.org/10.4331/wjbc.v6.i4.366 |
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